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Literature summary extracted from

  • Ishikura, S.; Usami, N.; Araki, M.; Hara, A.
    Structural and functional characterization of rabbit and human L-gulonate 3-dehydrogenase (2005), J. Biochem., 137, 303-314.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.45 expressed in Escherichia coli Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.45 D36R mutation leads to a switch in the favor of NADP(H) specificity, suggesting an important role of Asp36 in the coenzyme specificity Oryctolagus cuniculus
1.1.1.45 E157Q mutation produces no large kinetic alterations Oryctolagus cuniculus
1.1.1.45 H145Q inactive enzyme form Oryctolagus cuniculus
1.1.1.45 N195D inactive enzyme form Oryctolagus cuniculus
1.1.1.45 N196Q inactive enzyme form Oryctolagus cuniculus
1.1.1.45 S124A mutation decreases the catalytic efficiency 500 fold Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.45 Cibacron blue competitive inhibition with respect to NAD+ Oryctolagus cuniculus
1.1.1.45 malonate reversible inhibition, IC50: 0.34 mM Oryctolagus cuniculus
1.1.1.45 malonic acid monoethyl ester weak inhibition, IC50: 5.5 mM Oryctolagus cuniculus
1.1.1.45 additional information no inhibition by 20 mM D-glucose, 2 mM 2-keto-L-gulonate, 1 mM D-glucorunate or 6 mM ADP, ATP and AMP; no significant inhibition (less than 15%) by 1 mM L-ascorbate, monocarboxylic acids, or dicarboxylic acids Oryctolagus cuniculus
1.1.1.45 p-chloromercuriphenylsulfonate 0.001 mM, complete inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.45 additional information
-
 additional information no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate Oryctolagus cuniculus
1.1.1.45 0.0005
-
 NADH at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 0.0006
-
 NADH at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 0.01
-
 NAD+ at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 0.011
-
 NAD+ at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 0.18
-
 L-gulonate at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 0.21
-
 L-gulonate at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 0.49
-
 L-gulonate mutant enzyme E157Q, NAD+-linked activity Oryctolagus cuniculus
1.1.1.45 0.67
-
 NADP+ at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 0.89
-
 NADP+ at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 1.2
-
 L-threonate at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 2.2
-
 L-3-hydroxybutyrate at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 2.3
-
 L-3-hydroxybutyrate at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 2.9
-
 L-threonate at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 4.4
-
 L-gulonate mutant enzyme S124A, NAD+-linked activity Oryctolagus cuniculus
1.1.1.45 8.3
-
 acetoacetate at pH 8.5, rabbit liver GDH Oryctolagus cuniculus
1.1.1.45 9.9
-
 acetoacetate at pH 8.5, recombinant rabbit GDH Oryctolagus cuniculus
1.1.1.45 14
-
 L-gulonate mutant enzyme D36R, NAD+-linked activity Oryctolagus cuniculus
1.1.1.45 18.5
-
 L-gulonate mutant enzyme D36R, NADP+-linked activity Oryctolagus cuniculus
1.1.1.45 18.5
-
 L-gulonate mutant enzyme E157Q, NADP+-linked activity Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.45 cytosol
-
 Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.45 35000
-
 2 * 35000 Oryctolagus cuniculus
1.1.1.45 36000
-
 SDS-PAGE Oryctolagus cuniculus
1.1.1.45 70000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.45 Oryctolagus cuniculus
-
 Japanese white rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.45 ammonium sulfate fractionation, Sephadex G-100 column chromatography, Q-Sepharose column chromatography, Blue-Sepharose column chromatography, hydroxylapatite column chromatography Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.45 lens
-
 Oryctolagus cuniculus
-
1.1.1.45 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.45 0.011
-
 in the supernatant Oryctolagus cuniculus
1.1.1.45 6.71
-
 after purification Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.45 ? + NADP+
-
 Oryctolagus cuniculus ? + NADPH
-
 ?
1.1.1.45 acetoacetate + NADH
-
 Oryctolagus cuniculus ? + NAD+
-
 ?
1.1.1.45 L-3-hydroxybutyrate + ?
-
 Oryctolagus cuniculus ?
-
 ?
1.1.1.45 L-gulonate + NAD+
-
 Oryctolagus cuniculus 3-dehydro-L-gulonate + NADH + H+
-
 ?
1.1.1.45 L-threonate + ?
-
 Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.45 dimer 2 * 35000 Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.45 GDH
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.45 6.5
-
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.45 NADH
-
 Oryctolagus cuniculus
1.1.1.45 NADP+
-
 Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.45 Oryctolagus cuniculus
-
-
 7.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.45 0.34
-
 reversible inhibition, IC50: 0.34 mM Oryctolagus cuniculus malonate
1.1.1.45 5.5
-
 weak inhibition, IC50: 5.5 mM Oryctolagus cuniculus malonic acid monoethyl ester