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Literature summary extracted from

  • Matsumiya, Y.; Nishikawa, K.; Aoshima, H.; Inouye, K.; Kubo, M.
    Analysis of autodegradation sites of thermolysin and enhancement of its thermostability by modifying Leu155 at an autodegradation site (2004), J. Biochem., 135, 547-553.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.27 L155A thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to A155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
3.4.24.27 L155F thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to F155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
3.4.24.27 L155G thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to G155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
3.4.24.27 L155S thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to S155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 10
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155G, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 13
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155S, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 14
-
 N-carbobenzoxy-Gly-L-Leu-NH2 wild-type, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 17
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155F, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 20
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155A, 37°C, pH 7.0 Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 N-carbobenzoxy-Gly-L-Leu-NH2 + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.27 More in presence of Ca2+, enzyme exhibits four autodegradation sites, one of them being Gly154-Leu155 Bacillus thermoproteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 23.3
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155F, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 51.6
-
 N-carbobenzoxy-Gly-L-Leu-NH2 wild-type, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 80
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155A, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 150
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155G, 37°C, pH 7.0 Bacillus thermoproteolyticus
3.4.24.27 165
-
 N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155S, 37°C, pH 7.0 Bacillus thermoproteolyticus