EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.27 | L155A | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to A155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
3.4.24.27 | L155F | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to F155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
3.4.24.27 | L155G | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to G155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
3.4.24.27 | L155S | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to S155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 10 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155G, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 13 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155S, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 14 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | wild-type, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 17 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155F, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 20 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155A, 37°C, pH 7.0 | Bacillus thermoproteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.27 | Bacillus thermoproteolyticus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.27 | N-carbobenzoxy-Gly-L-Leu-NH2 + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.27 | More | in presence of Ca2+, enzyme exhibits four autodegradation sites, one of them being Gly154-Leu155 | Bacillus thermoproteolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 23.3 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155F, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 51.6 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | wild-type, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 80 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155A, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 150 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155G, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
3.4.24.27 | 165 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155S, 37°C, pH 7.0 | Bacillus thermoproteolyticus |