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Literature summary extracted from

  • Carroll, T.M.; Setlow, P.
    Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease (2005), J. Bacteriol., 187, 7119-7125.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.24.78 degradation D127 and D193 are essential for activity and autoprocessing Priestia megaterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.78 subcloned into the pET23a vector, overexpression of full-length gene P46 and a truncated form encoding only P41 in Escherichia coli Bl21(DE3)pLysS Priestia megaterium

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.78 D127A no activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D127E no activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D127N no activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D127S no activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D153N activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 D193N no activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D211N activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 D246N partial activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 D253N activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 D256N activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 E201Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 E26Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 E89Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 H125Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 H142Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 K21P activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 K223A activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 K223E activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 K223H activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 K223M activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 additional information Delta223 mutant with activity of P41 against small acid-soluble spore protein and autoprocessing of P46, Delta213-226 mutant, Delta213-227 mutant, Delta214-226 mutant and Delta214-227 mutant with no activity of P41 against small acid-soluble spore protein and no autoprocessing of P46 Priestia megaterium
3.4.24.78 Q208E activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 R202Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 R224Q activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 S199A activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 S210A activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 S212A activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 S218A activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 S249A partial activity of P41 against small acid-soluble spore protein, no autoprocessing of P46 Priestia megaterium
3.4.24.78 T125N activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 T173V activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 T215V activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 T74D activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium
3.4.24.78 Y72F activity of P41 against small acid-soluble spore protein, autoprocessing of P46 Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.78 Priestia megaterium
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.78 small acid-soluble protein + H2O
-
 Priestia megaterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.78 tetramer wild-type P46 nearly 100% tetrameric, P41 largely tetrameric but also with a significant percentage of lower-molecular-mass-species Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
3.4.24.78 germination proteinase
-
 Priestia megaterium
3.4.24.78 GPR
-
 Priestia megaterium