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Literature summary extracted from

  • Ko, J.; Kim, I.; Yoo, S.; Min, B.; Kim, K.; Park, C.
    Conversion of methylglyoxal to acetol by Escherichia coli aldo-keto reductases (2005), J. Bacteriol., 187, 5782-5789.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.274 2.05
-
 methylglyoxal YqhE Escherichia coli
1.1.1.274 2.46
-
 methylglyoxal YafB Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.274 DEAE-Sepharose column chromatography and Superdex 200 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.274 0.01
-
 YafB, 1 mM 2-carboxybenzaldehyde as substrate Escherichia coli
1.1.1.274 0.01
-
 YafB, 10 mM D-xylose as substrate Escherichia coli
1.1.1.274 0.01
-
 YqhE, 1 mM 2-carboxybenzaldehyde as substrate Escherichia coli
1.1.1.274 0.0153
-
 YafB, 10 mM D-glucuronic acid as substrate Escherichia coli
1.1.1.274 0.0247
-
 YqhE, 10 mM D-glucuronic acid as substrate Escherichia coli
1.1.1.274 0.0401
-
 YafB, 1 mM 2,5-diketo-D-gluconate as substrate Escherichia coli
1.1.1.274 0.0424
-
 YafB, 0.1 mM 1,2-naphthoquinone as substrate Escherichia coli
1.1.1.274 0.0519
-
 YqhE, 1 mM 2,5-diketo-D-gluconate as substrate Escherichia coli
1.1.1.274 0.0949
-
 YqhE, 10 mM D-xylose as substrate Escherichia coli
1.1.1.274 0.127
-
 YqhE, 0.1 mM 1,2-naphthoquinone as substrate Escherichia coli
1.1.1.274 1.36
-
 YafB, 1 mM DL-glyceraldehyde as substrate Escherichia coli
1.1.1.274 2.21
-
 YqhE, 1 mM DL-glyceraldehyde as substrate Escherichia coli
1.1.1.274 2.23
-
 YafB, 1 mM 3-nitrobenzaldehyde as substrate Escherichia coli
1.1.1.274 2.5
-
 YqhE, 1 mM 3-nitrobenzaldehyde as substrate Escherichia coli
1.1.1.274 2.79
-
 YafB, 1 mM benzaldehyde as substrate Escherichia coli
1.1.1.274 3.88
-
 YqhE, 1 mM benzaldehyde as substrate Escherichia coli
1.1.1.274 6.53
-
 YafB, 1 mM 4-nitrobenzaldehyde as substrate Escherichia coli
1.1.1.274 10.3
-
 YqhE, 1 mM 4-nitrobenzaldehyde as substrate Escherichia coli
1.1.1.274 14.9
-
 YafB, 1 mM methylglyoxal as substrate Escherichia coli
1.1.1.274 21
-
 YqhE, 1 mM methylglyoxal as substrate Escherichia coli
1.1.1.274 39.1
-
 YafB, 1 mM phenylglyoxal as substrate Escherichia coli
1.1.1.274 64.4
-
 YqhE, 1 mM phenylglyoxal as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.274 1,2-naphthoquinone + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
1.1.1.274 2,5-diketo-D-gluconate + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
1.1.1.274 2-carboxybenzaldehyde + NADPH
-
 Escherichia coli 2-hydroxybenzoate + NADP+
-
 ?
1.1.1.274 3-nitrobenzaldehyde + NADPH
-
 Escherichia coli 3-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.274 4-nitrobenzaldehyde + NADPH
-
 Escherichia coli 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.274 benzaldehyde + NADPH
-
 Escherichia coli benzyl alcohol + NADP+
-
 ?
1.1.1.274 D-glucuronic acid + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
1.1.1.274 D-xylose + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
1.1.1.274 DL-glyceraldehyde + NADPH + H+
-
 Escherichia coli glycerol + NADP+
-
 ?
1.1.1.274 methylglyoxal + NADPH
-
 Escherichia coli ? + NADP+
-
 ?
1.1.1.274 phenylglyoxal + NADPH
-
 Escherichia coli ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.274 2,5-diketo-gluconate reductase
-
 Escherichia coli
1.1.1.274 2,5-DKG
-
 Escherichia coli
1.1.1.274 YafB
-
 Escherichia coli
1.1.1.274 YqhE
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.274 27.62
-
 methylglyoxal YqhE Escherichia coli
1.1.1.274 29.15
-
 methylglyoxal YafB Escherichia coli
1.1.1.274 81
-
 methylglyoxal YafB Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.274 NADPH
-
 Escherichia coli