EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.19 | 3,4-dichloroisocoumarin | 0.3 mM, 62% inhibition of isoform -1S-SprE | Enterococcus faecalis | |
3.4.21.19 | diisopropyl fluorophosphate | 0.3 mM, complete inhibition of isoform -1S-SprE | Enterococcus faecalis | |
3.4.21.19 | additional information | not inhibitory: E-64, pepstatin, o-phenanthroline, EDTA | Enterococcus faecalis | |
3.4.21.19 | NaCl | 1 mM, 80% inhibition of isoform -1S-SprE | Enterococcus faecalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.19 | 1.027 | - |
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide | isoform -1S-SprE, pH 7.5, 37°C | Enterococcus faecalis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.21.19 | extracellular | - |
Enterococcus faecalis | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.19 | Enterococcus faecalis | Q47809 | - |
- |
3.4.21.19 | Enterococcus faecalis OG1RF | Q47809 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.21.19 | proteolytic modification | proteolytic modification of zymogen dependent on the presence of gelatinase. In absence of gelatinase, maturation to different isoforms including a highly active isoform -1S-SprE with protein sequence Ser-1 to Glu227 | Enterococcus faecalis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.19 | 20140 | - |
isoform -1S-SprE, pH 7.5, 37°C | Enterococcus faecalis |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.19 | -70°C, all isoforms stable for at least 6 months | Enterococcus faecalis |
3.4.21.19 | 4°C, isoform -1S-SprE, 50% loss of activity within 4 weeks, isoform 1l-SprE, stable | Enterococcus faecalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.19 | benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O | 35% of the efficiency with succinyl-Ala-Ala-Pro-Glu-p-nitroanilide | Enterococcus faecalis | benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline | - |
? | |
3.4.21.19 | benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O | 35% of the efficiency with succinyl-Ala-Ala-Pro-Glu-p-nitroanilide | Enterococcus faecalis OG1RF | benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline | - |
? | |
3.4.21.19 | succinyl-Ala-Ala-Pro-Glu-p-nitroanilide + H2O | - |
Enterococcus faecalis | succinyl-Ala-Ala-Pro-Glu + p-nitroaniline | - |
? | |
3.4.21.19 | succinyl-Ala-Ala-Pro-Glu-p-nitroanilide + H2O | - |
Enterococcus faecalis OG1RF | succinyl-Ala-Ala-Pro-Glu + p-nitroaniline | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.19 | 7 | 7.5 | isoform -1S-SprE, inversely dependent on the ionic strength of buffer | Enterococcus faecalis |