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Literature summary extracted from

  • Park, H.S.; Kim, H.S.
    Identification and characterization of the nitrobenzene catabolic plasmids pNB1 and pNB2 in Pseudomonas putida HS12 (2000), J. Bacteriol., 182, 573-580.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.99.5 molecular biology nbzE, involved in the ring cleavage pathway of 2-aminophenol, is localized on the 6.6 kb SnaBI-SmaI fragment of the plasmid pNB1 and clusters in the order nbzC-nbzD-nbzE as an operon Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.5 cloned into plasmid pBR328, expression in Escherichia coli JM109 harboring plasmids pHS29, pHS293, pHS295, pHS291, pHS2915 and PHS2916 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
3.5.99.5 additional information spontaneous mutants HS121(pNB2) during serial transfer on nonselective agar plates, can not grow on nitrobenzene as the sole source of carbon, nitrogen and energy, have lost plasmid pNB1, plasmid-cured HS124 mutant, obtained by incubating the parent strain at an elevated temperature, have lost plasmid pNB2 and also can not grow on nitrobenzene Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.5 Pseudomonas putida
-
-
-
3.5.99.5 Pseudomonas putida HS12
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.5 2-aminomuconate + H2O part of the nitrobenzene degradation pathway Pseudomonas putida 4-oxalocrotonate + NH3
-
 ?
3.5.99.5 2-aminomuconate + H2O part of the nitrobenzene degradation pathway Pseudomonas putida HS12 4-oxalocrotonate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.99.5 2-AM deaminase
-
 Pseudomonas putida
3.5.99.5 2-aminomuconate deaminase
-
 Pseudomonas putida
3.5.99.5 nbzE
-
 Pseudomonas putida