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Literature summary extracted from

  • Katayama, Y.; Hashimoto, K.; Nakayama, H.; Mino, H.; Nojiri, M.; Ono, T.; Nyunoya, H.; Yohda, M.; Takio, K.; Odaka, M.
    Thiocyanate hydrolase is a cobalt-containing metalloenzyme with a cysteine-sulfinic acid ligand (2006), J. Am. Chem. Soc., 128, 728-729.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.8 DNA and amino acid sequence determination and analysis Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.8 Co2+ cobalt-containing metalloenzyme, 1 Co2+ per enzyme trimer, binding site, H124VVVCTLCSCYPRPILGQSPEWYR147, in the gamma-subunit with close relation to nitrile hydratase, absorption spectrum analysis, overview Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.8 thiocyanate + H2O Thiobacillus thioparus
-
 carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O Thiobacillus thioparus THI115
-
 carbonyl sulfide + NH3 + OH-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Thiobacillus thioparus
-
-
-
3.5.5.8 Thiobacillus thioparus THI115
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.5.8 additional information Cys113 is posttranslationally oxidized to cysteinesulfinic acid Thiobacillus thioparus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.8 additional information the organism is obligately chemilithoautotrophic sulfur-oxidizing and uses thiocyanate as sole energy source Thiobacillus thioparus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O via multiple reaction steps Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O via multiple reaction steps Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + OH-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.8 trimer heterotrimer alphabetagamma Thiobacillus thioparus

Synonyms

EC Number Synonyms Comment Organism
3.5.5.8 SCNase
-
 Thiobacillus thioparus