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Literature summary extracted from

  • Wu, P.C.; Liu, Y.H.; Wang, Z.Y.; Zhang, X.Y.; Li, H.; Liang, W.Q.; Luo, N.; Hu, J.M.; Lu, J.Q.; Luan, T.G.; Cao, L.X.
    Molecular cloning, purification, and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112 (2006), J. Agric. Food Chem., 54, 836-842.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1
-
 Klebsiella sp.
3.1.1.1 expression in Escherichia coli Klebsiella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Ag+ 0.5 mM, complete inhibition; strong Klebsiella sp.
3.1.1.1 Hg2+ 0.5 mM, complete inhibition; strong Klebsiella sp.
3.1.1.1 additional information 1 mM EDTA or 1 mM phenanthroline show little effect on activity Klebsiella sp.
3.1.1.1 p-chloromercuribenzoate 0.1 mM, strong inhibition; strong Klebsiella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.00011
-
 trans-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.00016
-
 cis-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.00021
-
 cypermethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.00087
-
 malathion pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.00091
-
 fenvalerate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.00123
-
 deltamethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.037
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.037
-
 4-nitrophenyl caproate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.068
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.068
-
 4-nitrophenyl caprylate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.092
-
 4-nitrophenyl butyrate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.092
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.139
-
 4-nitrophenyl propionate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.139
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.261
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.261
-
 p-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.339
-
 4-nitrophenyl laurate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.339
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 72000
-
 gel filtration Klebsiella sp.
3.1.1.1 73000
-
 gel filtration Klebsiella sp.
3.1.1.1 73500
-
 1 * 73000, SDS-PAGE, 1 * 73500, calculated Klebsiella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Klebsiella sp. Q52NW7
-
-
3.1.1.1 Klebsiella sp. Q52NW7 strain ZD112
-
3.1.1.1 Klebsiella sp. ZD112 Q52NW7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Klebsiella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 0.53
-
 substrate p-nitrophenyl deltamethrin, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 2.4
-
 substrate p-nitrophenyl malathion, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 3.5
-
 substrate p-nitrophenyl fenvalerate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 9.8
-
 substrate p-nitrophenyl cypermethrin, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 35.6
-
 substrate p-nitrophenyl cis-permethrin, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 38.8
-
 substrate p-nitrophenyl trans-permethrin, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 67
-
 substrate p-nitrophenyl laurate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 91
-
 substrate p-nitrophenyl acetate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 246.6
-
 substrate: 4-nitrophenyl acetate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 269
-
 substrate p-nitrophenyl propionate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 355
-
 substrate p-nitrophenyl butyrate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 407
-
 substrate p-nitrophenyl caprylate, pH 7.0, 30°C Klebsiella sp.
3.1.1.1 573
-
 substrate p-nitrophenyl caproate, pH 7.0, 30°C Klebsiella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Klebsiella sp. 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Klebsiella sp. 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Klebsiella sp. 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O
-
 Klebsiella sp. 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O
-
 Klebsiella sp. 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O
-
 Klebsiella sp. 4-nitrophenol + laurate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O medium-chain 4-nitrophenyl esters are the preferred substrates, whereas 4-nitrophenyl esters of longer chain fatty acids are poor substrates Klebsiella sp. 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl propanoate + H2O
-
 Klebsiella sp. 4-nitrophenol + propanoate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Klebsiella sp. 4-nitrophenol + propionate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Klebsiella sp. ZD112 4-nitrophenol + propionate
-
 ?
3.1.1.1 cis-permethrin + H2O
-
 Klebsiella sp. ?
-
 ?
3.1.1.1 cypermethrin + H2O
-
 Klebsiella sp. ?
-
 ?
3.1.1.1 deltamethrin + H2O
-
 Klebsiella sp. 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.1 fenvalerate + H2O
-
 Klebsiella sp. ?
-
 ?
3.1.1.1 malathion + H2O
-
 Klebsiella sp. ?
-
 ?
3.1.1.1 additional information no substrate: 4-nitrophenyl myristate, 4-nitrophenol + palmitate Klebsiella sp. ?
-
 ?
3.1.1.1 additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Klebsiella sp. ?
-
 ?
3.1.1.1 additional information no substrate: 4-nitrophenyl myristate, 4-nitrophenol + palmitate Klebsiella sp. ZD112 ?
-
 ?
3.1.1.1 additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Klebsiella sp. ZD112 ?
-
 ?
3.1.1.1 trans-permethrin + H2O
-
 Klebsiella sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 73000, SDS-PAGE Klebsiella sp.
3.1.1.1 monomer 1 * 73000, SDS-PAGE, 1 * 73500, calculated Klebsiella sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EstP
-
 Klebsiella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 40
-
 substrate: 4-nitrophenyl acetate Klebsiella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 45
-
 1 h, pH 7.0, stable up to Klebsiella sp.
3.1.1.1 50
-
 1 h, pH 7.0, 46% loss of activity Klebsiella sp.
3.1.1.1 65
-
 1 h, pH 7.0, complete inactivation Klebsiella sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 0.016
-
 deltamethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.07
-
 malathion pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.103
-
 fenvalerate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 0.288
-
 cypermethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 1.05
-
 cis-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 1.14
-
 trans-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.1 59
-
 4-nitrophenyl laurate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 59
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 81
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 81
-
 p-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 238
-
 4-nitrophenyl propionate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 238
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 314
-
 4-nitrophenyl butyrate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 314
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 360
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 360
-
 4-nitrophenyl caprylate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 507
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 507
-
 4-nitrophenyl caproate pH 7.0, 30°C Klebsiella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
 substrate: 4-nitrophenyl acetate Klebsiella sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 5.5 9 2 h, 30°C, stable Klebsiella sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Klebsiella sp. isoelectric focusing, pH-range: 3.5-10.0
-
 8.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 170
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 310
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 1710
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 3410
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 5300
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.1 13700
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 13
-
 deltamethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.88 113
-
 fenvalerate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 170
-
 4-nitrophenyl dodecanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 310
-
 4-nitrophenyl acetate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 810
-
 malathion pH 7.0, 30°C Klebsiella sp.
3.1.1.88 1370
-
 cypermethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.88 1710
-
 4-nitrophenyl propanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 3410
-
 4-nitrophenyl butanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 5300
-
 4-nitrophenyl octanoate pH 7.0, 30°C Klebsiella sp.
3.1.1.88 6560
-
 cis-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.88 10360
-
 trans-permethrin pH 7.0, 30°C Klebsiella sp.
3.1.1.88 13700
-
 4-nitrophenyl hexanoate pH 7.0, 30°C Klebsiella sp.