EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.37 | mitochondrial and glycosomal malate dehydrogenase isoenzyme, functional expression in Escherichia coli | Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.37 | 0.015 | - |
oxaloacetate | glycosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.036 | - |
NADH | glycosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.054 | - |
NADH | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.069 | - |
oxaloacetate | glycosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.085 | - |
NADH | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.32 | - |
NADH | glacosomal enzyme | Trypanosoma brucei | |
1.1.1.37 | 0.56 | - |
(S)-malate | pH 7.5, 37°C, mitochondrial enzyme | Trypanosoma brucei | |
1.1.1.37 | 2.7 | - |
p-hydroxyphenylpyruvate | - |
Trypanosoma brucei | |
1.1.1.37 | 2.72 | - |
(S)-malate | pH 7.5, 37°C, glycosomal enzyme | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.37 | cytosol | cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form | Trypanosoma brucei | 5829 | - |
1.1.1.37 | glycosome | - |
Trypanosoma brucei | 20015 | - |
1.1.1.37 | mitochondrion | - |
Trypanosoma brucei | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + NAD+ | Trypanosoma brucei | mitochondrial enzyme, Krebs cycle | oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH | Trypanosoma brucei | cytosolic enzyme and glycosomal enzyme | (S)-malate + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.37 | Trypanosoma brucei | O15769 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.37 | bloodstream form | the cytosolic isoenzyme is the only isoform of malate dehydrogenase expressed in the bloodstream form | Trypanosoma brucei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.37 | (S)-malate + NAD+ | mitochondrial enzyme, Krebs cycle | Trypanosoma brucei | oxaloacetate + NADH + H+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH | cytosolic enzyme and glycosomal enzyme | Trypanosoma brucei | (S)-malate + NAD+ | - |
r | |
1.1.1.37 | oxaloacetate + NADH + H+ | - |
Trypanosoma brucei | (S)-malate + NAD+ | - |
r | |
1.1.1.37 | p-hydroxyphenylpyruvate + NADH + H+ | - |
Trypanosoma brucei | 2-hydroxy-3-(4-hydroxyphenyl)propanoate | - |
? | |
1.1.1.37 | phenylpyruvate + NADH + H+ | - |
Trypanosoma brucei | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? |