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Literature summary extracted from
- Refolding intermediate of guanidine hydrochloride denatured aminoacylase (2004), Int. J. Biochem. Cell Biol., 36, 1332-1340.
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.14 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration to homogeneity | Sus scrofa |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | denaturation of the enzyme with 6 M guanidinium hydrochloride, dilution 60fold with Tris-HCl buffer, pH 7.5, determination of the refolding intermediate of guanidine hydrochloride denatured aminoacylase, the refolded enzyme shows 58-24% of maximal activity dependent on the temperature during refolding, 58% of native activity after refolding at 4°C, overview | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.14 | kidney | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.14 | chloroacetyl-L-leucine + H2O | - |
Sus scrofa | chloroacetate + L-leucine | - |
r |  |
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Release 2023.1 (January 2023)
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