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Literature summary extracted from

  • Matsuda, Y.; Kubo, T.
    Gametolysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 592-595.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.38 degradation degrades gametic cell walls, cell walls of vegetative cells and those of mother sporangial cells Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.38
-
 Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.38 1,10-phenanthroline
-
 Chlamydomonas reinhardtii
3.4.24.38 Alpha-macroglobulin
-
 Chlamydomonas reinhardtii
3.4.24.38 EDTA
-
 Chlamydomonas reinhardtii
3.4.24.38 EGTA
-
 Chlamydomonas reinhardtii
3.4.24.38 HgCl2
-
 Chlamydomonas reinhardtii
3.4.24.38 p-chloromercuribenzoic acid
-
 Chlamydomonas reinhardtii
3.4.24.38 phosphoramidon inhibits at a high concentration Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.38 periplasm proenzyme Chlamydomonas reinhardtii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.38 Ca2+ slightly stimulates at 0.75-1 mM Chlamydomonas reinhardtii
3.4.24.38 Mg2+ slightly stimulates at 0.75-1 mM Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.38 49630
-
 mature enzyme Chlamydomonas reinhardtii
3.4.24.38 60000 62000 active enzyme secreted into the mating medium Chlamydomonas reinhardtii
3.4.24.38 65000
-
 proenzyme Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.38 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.38 30-140fold Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.38 additional information mating gamete Chlamydomonas reinhardtii
-
3.4.24.38 vegetative cell proenzyme Chlamydomonas reinhardtii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.38 alpha-neoendorphin + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 dynorphin(1-13) + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 Mastoparan + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?
3.4.24.38 neurotensin + H2O
-
 Chlamydomonas reinhardtii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.38 cell wall lytic enzyme
-
 Chlamydomonas reinhardtii
3.4.24.38 Gamete autolysin
-
 Chlamydomonas reinhardtii
3.4.24.38 Gamete lytic enzyme
-
 Chlamydomonas reinhardtii
3.4.24.38 GLE
-
 Chlamydomonas reinhardtii
3.4.24.38 mmp1
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.38 35
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.38 7.5
-
-
 Chlamydomonas reinhardtii