Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takahashi, K.
    Acrocylindropepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 91-92.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.28 purified enzyme, repeated crystallization Acrocylindrium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.28 1,2-epoxy-3-(4-nitrophenoxy)propane i.e. EPNP Acrocylindrium sp.
3.4.23.28 Diazoacetyl-DL-norleucine methyl ester i.e. DAN, in presence of cupric ions Acrocylindrium sp.
3.4.23.28 pepstatin strong inhibition Acrocylindrium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.28 extracellular the enzyme is secreted Acrocylindrium sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.28 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + 6 H2O Acrocylindrium sp. i.e. insulin B chain, cleavage site specificity FVNQHL + CGSHL + VEAL + L-Tyr + LVCGERGF + L-Phe + YTPKA
-
 ?
3.4.23.28 Glucagon + H2O Acrocylindrium sp.
-
 ?
-
 ?
3.4.23.28 insulin A chain + H2O Acrocylindrium sp. cleavage site specificity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.28 Acrocylindrium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.28 native enzyme from cell culture medium by successive acetone precipitation, ammonium sulfate fractionation, and adsorption chromatography, to homogeneity Acrocylindrium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.28 additional information
-
-
 Acrocylindrium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.28 casein + H2O high activity Acrocylindrium sp. ?
-
 ?
3.4.23.28 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + 6 H2O i.e. insulin B chain, cleavage site specificity Acrocylindrium sp. FVNQHL + CGSHL + VEAL + L-Tyr + LVCGERGF + L-Phe + YTPKA
-
 ?
3.4.23.28 Glucagon + H2O
-
 Acrocylindrium sp. ?
-
 ?
3.4.23.28 insulin A chain + H2O cleavage site specificity Acrocylindrium sp. ?
-
 ?
3.4.23.28 additional information the enzyme prefers Tyr, Phe, or Leu at P1 or P1' positions Acrocylindrium sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.23.28 More the enzyme belongs to the A1 peptidase family Acrocylindrium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.28 37
-
 assay at Acrocylindrium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.28 50
-
 stable below Acrocylindrium sp.
3.4.23.28 70
-
 inactivation above Acrocylindrium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.28 2
-
 substrate casein Acrocylindrium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.28 2.5 5 highly stable, unstable below pH 0.7 and above pH 5.6 Acrocylindrium sp.