Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Foltmann, B.; Szecsi, P.B.
    Pepsin B (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 28-29.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.2 36000
-
 x * 41000, pepsinogen, SDS-PAGE, x * 36000, pepsin B, SDS-PAGE Sus scrofa
3.4.23.2 41000
-
 x * 41000, pepsinogen, SDS-PAGE, x * 36000, pepsin B, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.2 Gelatin + H2O Sus scrofa liquefication ?
-
 ?
3.4.23.2 additional information Sus scrofa the enzyme shows milk clotting activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.2 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.2 proteolytic modification pepssinogen B is proteolytically activated in the gastric tract, first cleavage site is Met16-Glu17, convertion at pH 5.5, not at pH 2.0 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.2 native pepsinogen by gel filtration and anion exchange chromatography to homogeneity Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.2 gastric mucosa production of pepsinogen Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.2 Ac-Phe-Tyr radio-labeled substrate Sus scrofa Ac-Phe + Tyr
-
 ?
3.4.23.2 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O oxidized insulin B chain, cleavage site specificity determination Sus scrofa FVNQHLCGSHL + VEA + Leu + Tyr + LVCGERGF + Phe + YTPKA
-
 ?
3.4.23.2 Gelatin + H2O liquefication Sus scrofa ?
-
 ?
3.4.23.2 Hemoglobin + H2O acid-denatured protein substrate Sus scrofa ?
-
 ?
3.4.23.2 additional information the enzyme shows milk clotting activity Sus scrofa ?
-
 ?
3.4.23.2 additional information the enzyme shows generally low proteolytic activity Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.2 ? x * 41000, pepsinogen, SDS-PAGE, x * 36000, pepsin B, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.23.2 More the enzyme belongs to the A1 peptidase family Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.2 3
-
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.2 4 6.9 room temperature, pepsin is stable Sus scrofa