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Literature summary extracted from
- Signal peptidase II (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 201-204.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.36 | detergent | required for activity, e.g. Triton X-100 or Nikkol, but not lauryl sarcoside or octylglucoside | Escherichia coli |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.36 | DNA and amino acid sequence determination and analysis | Staphylococcus aureus |
| 3.4.23.36 | DNA and amino acid sequence determination and analysis | Pseudomonas fluorescens |
| 3.4.23.36 | DNA and amino acid sequence determination and analysis | Klebsiella aerogenes |
| 3.4.23.36 | enzyme overexpression in Escherichia coli confers high resistance to globomycin | Myxococcus xanthus |
| 3.4.23.36 | gene lsp, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli under control of the trp promoter | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.36 | Globomycin | - |
Bacillus subtilis |  |
| 3.4.23.36 | Globomycin | uncompetitive | Escherichia coli | |
| 3.4.23.36 | Globomycin | - |
Klebsiella aerogenes | |
| 3.4.23.36 | Globomycin | - |
Myxococcus xanthus | |
| 3.4.23.36 | Globomycin | - |
Pseudomonas fluorescens | |
| 3.4.23.36 | Globomycin | - |
Staphylococcus aureus | |
| 3.4.23.36 | HgCl2 | - |
Escherichia coli | |
| 3.4.23.36 | lauroyl sarcoside | - |
Escherichia coli | |
| 3.4.23.36 | octylglucoside | - |
Escherichia coli | |
| 3.4.23.36 | tosyl arginyl methyl ester | - |
Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.23.36 | inner membrane | - |
Staphylococcus aureus | - |
- |
| 3.4.23.36 | inner membrane | - |
Bacillus subtilis | - |
- |
| 3.4.23.36 | inner membrane | - |
Pseudomonas fluorescens | - |
- |
| 3.4.23.36 | inner membrane | - |
Klebsiella aerogenes | - |
- |
| 3.4.23.36 | inner membrane | - |
Myxococcus xanthus | - |
- |
| 3.4.23.36 | inner membrane | transmembrane protein with a large and a small loop at the periplasmic side | Escherichia coli | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Staphylococcus aureus | |
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Bacillus subtilis | |
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Escherichia coli | |
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Pseudomonas fluorescens | |
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Klebsiella aerogenes | |
| 3.4.23.36 | additional information | no requirement for metal ions, thiol reagents, and phospholipids | Myxococcus xanthus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.36 | 18000 | - |
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
| 3.4.23.36 | 18144 | - |
x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.36 | bacterial lipoprotein + H2O | Staphylococcus aureus | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | Bacillus subtilis | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | Escherichia coli | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | Pseudomonas fluorescens | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | Klebsiella aerogenes | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | Myxococcus xanthus | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.36 | Bacillus subtilis | - |
- |
- |
| 3.4.23.36 | Escherichia coli | - |
gene lsp | - |
| 3.4.23.36 | Klebsiella aerogenes | - |
- |
- |
| 3.4.23.36 | Myxococcus xanthus | - |
- |
- |
| 3.4.23.36 | Pseudomonas fluorescens | - |
- |
- |
| 3.4.23.36 | Staphylococcus aureus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.36 | additional information | N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme | Escherichia coli |
| 3.4.23.36 | proteolytic modification | N-terminal Cys modification with diacylglyceryl before cleavage of the signal peptide to the mature enzyme | Escherichia coli |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.36 | native enzyme from inner membrane to homogeneity by solubilization from membrane with Triton X-100, heat treatmemt at 65°C and pH 4.0, anion exchange chromatography, and chromatofocusing, 35000fold, recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography in presence of EDTA, and gel filtration to homogeneity | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Staphylococcus aureus | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Bacillus subtilis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Escherichia coli | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Pseudomonas fluorescens | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Klebsiella aerogenes | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide, the enzyme is essential for lipoprotein biosynthesis | Myxococcus xanthus | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Staphylococcus aureus | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Bacillus subtilis | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Escherichia coli | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Pseudomonas fluorescens | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Klebsiella aerogenes | ? | - |
? | |
| 3.4.23.36 | bacterial lipoprotein + H2O | cleavage of the signal peptide | Myxococcus xanthus | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Staphylococcus aureus | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Bacillus subtilis | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Escherichia coli | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Pseudomonas fluorescens | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Klebsiella aerogenes | ? | - |
? | |
| 3.4.23.36 | Braun's polylipoprotein + H2O | cleavage of the signal peptide, cleavage site specificity, overview | Myxococcus xanthus | ? | - |
? | |
| 3.4.23.36 | additional information | active site catalytic residues are Asp102 and Asp129 | Bacillus subtilis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.36 | ? | x * 18144, amino acid sequence calculation, x * 18000, SDS-PAGE | Escherichia coli |
| 3.4.23.36 | More | primary structure | Staphylococcus aureus |
| 3.4.23.36 | More | primary structure | Escherichia coli |
| 3.4.23.36 | More | primary structure | Pseudomonas fluorescens |
| 3.4.23.36 | More | primary structure | Klebsiella aerogenes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.36 | lipoprotein-specific signal peptidase | - |
Escherichia coli |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Staphylococcus aureus |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Bacillus subtilis |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Escherichia coli |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Pseudomonas fluorescens |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Klebsiella aerogenes |
| 3.4.23.36 | More | the enzyme belongs to the A8 peptidase family | Myxococcus xanthus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.36 | 80 | - |
purified enzyme, stable for a short time | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.36 | additional information | - |
neutral pH optimum | Staphylococcus aureus |
| 3.4.23.36 | additional information | - |
neutral pH optimum | Bacillus subtilis |
| 3.4.23.36 | additional information | - |
neutral pH optimum | Pseudomonas fluorescens |
| 3.4.23.36 | additional information | - |
neutral pH optimum | Klebsiella aerogenes |
| 3.4.23.36 | additional information | - |
neutral pH optimum | Myxococcus xanthus |
| 3.4.23.36 | 6 | - |
purified enzyme | Escherichia coli |
| 3.4.23.36 | 7.9 | - |
crude enzyme | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.36 | additional information | no requirement for cofactors | Staphylococcus aureus | |
| 3.4.23.36 | additional information | no requirement for cofactors | Bacillus subtilis | |
| 3.4.23.36 | additional information | no requirement for cofactors | Escherichia coli | |
| 3.4.23.36 | additional information | no requirement for cofactors | Pseudomonas fluorescens | |
| 3.4.23.36 | additional information | no requirement for cofactors | Klebsiella aerogenes | |
| 3.4.23.36 | additional information | no requirement for cofactors | Myxococcus xanthus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.36 | 0.000036 | - |
Globomycin | - |
Escherichia coli | |
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