EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.23.26 | purified native enzyme, 3times crystallization | Rhodotorula glutinis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.23.26 | 4-bromo-phenacylbromide | - |
Rhodotorula glutinis | |
3.4.23.26 | diazoacetyl glycine ethyl ester | - |
Rhodotorula glutinis | |
3.4.23.26 | Diazoacetyl-DL-norleucine methyl ester | i.e. DAN | Rhodotorula glutinis | |
3.4.23.26 | pepstatin Ac | 60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature | Rhodotorula glutinis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.23.26 | extracellular | - |
Rhodotorula glutinis | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.23.26 | 29000 | - |
gel filtration | Rhodotorula glutinis |
3.4.23.26 | 30000 | - |
sedimentation equilibrium analysis | Rhodotorula glutinis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.23.26 | Rhodotorula glutinis | - |
isolated from soil | - |
3.4.23.26 | Rhodotorula glutinis K-24 | - |
isolated from soil | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.23.26 | native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity | Rhodotorula glutinis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.23.26 | additional information | - |
- |
Rhodotorula glutinis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.23.26 | casein + H2O | high activity | Rhodotorula glutinis | ? | - |
? | |
3.4.23.26 | casein + H2O | high activity | Rhodotorula glutinis K-24 | ? | - |
? | |
3.4.23.26 | DRVYIHPFHLLVYS + H2O | tetradecapaptide renin substrate, cleavage pattern, overview | Rhodotorula glutinis | DRVY + IHPFHLL + VYS | - |
? | |
3.4.23.26 | DRVYIHPFHLLVYS + H2O | tetradecapaptide renin substrate, cleavage pattern, overview | Rhodotorula glutinis K-24 | DRVY + IHPFHLL + VYS | - |
? | |
3.4.23.26 | additional information | substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala | Rhodotorula glutinis | ? | - |
? | |
3.4.23.26 | additional information | substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala | Rhodotorula glutinis K-24 | ? | - |
? | |
3.4.23.26 | Z-Lys-Ala-Ala-Ala + H2O | - |
Rhodotorula glutinis | Z-Lys + Ala-Ala-Ala | - |
? | |
3.4.23.26 | Z-Lys-Ala-Ala-Ala + H2O | - |
Rhodotorula glutinis K-24 | Z-Lys + Ala-Ala-Ala | - |
? | |
3.4.23.26 | Z-Lys-Leu-Ala-Ala + H2O | - |
Rhodotorula glutinis | Z-Lys + Leu-Ala-Ala | - |
? | |
3.4.23.26 | Z-Lys-Leu-Ala-Ala + H2O | - |
Rhodotorula glutinis K-24 | Z-Lys + Leu-Ala-Ala | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.23.26 | 2 | 2.5 | - |
Rhodotorula glutinis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.23.26 | Rhodotorula glutinis | isoelectric focusing | - |
4.5 |