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Literature summary extracted from
- Schistosome legumain (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1305-1310.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.34 | expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
| 3.4.22.34 | expression of C197 in Pichia pastoris, expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | E64 | slight inhibition at 1 mM | Schistosoma mansoni |  |
| 3.4.22.34 | iodoacetamide | - |
Schistosoma mansoni | |
| 3.4.22.34 | leupeptin | slight inhibition at 1 mM | Schistosoma mansoni | |
| 3.4.22.34 | additional information | no inhibition by Z-Phe-Ala-CHN2 and 1,10-phenanthroline | Schistosoma mansoni | |
| 3.4.22.34 | N-ethylmaleimide | - |
Schistosoma mansoni | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.34 | extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma mansoni | - |
- |
| 3.4.22.34 | extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma japonicum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | additional information | no effect by DTT | Schistosoma mansoni |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 31000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| 3.4.22.34 | 32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| 3.4.22.34 | 32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
| 3.4.22.34 | 50000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| 3.4.22.34 | 50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| 3.4.22.34 | 50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | Hemoglobin + H2O | Schistosoma mansoni | the enzyme is involved in host hemoglobin degradation | ? | - |
? | |
| 3.4.22.34 | Hemoglobin + H2O | Schistosoma japonicum | the enzyme is involved in host hemoglobin degradation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | Homo sapiens | Q99538 | - |
- |
| 3.4.22.34 | Schistosoma japonicum | P42665 | the enzyme contains a Cys at position 197 | - |
| 3.4.22.34 | Schistosoma mansoni | P09841 | from infected mice and humans | - |
| 3.4.22.34 | Schistosoma mansoni | Q9NFY9 | fragment; from infected mice and humans | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | proteolytic modification | autoactivation | Schistosoma mansoni |
| 3.4.22.34 | proteolytic modification | autoactivation | Schistosoma japonicum |
| 3.4.22.34 | proteolytic modification | autoactivation at 37°C, pH 4.5 | Schistosoma mansoni |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.34 | native enzyme to homogeneity | Schistosoma mansoni |
| 3.4.22.34 | soluble recombinant C197 from Pichia pastoris, native enzyme to homogeneity | Schistosoma mansoni |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.22.34 | hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 | Schistosoma mansoni | |
| 3.4.22.34 | hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 and Cys197 | Schistosoma mansoni |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | additional information | activity only in adult worms | Schistosoma mansoni | - |
| 3.4.22.34 | additional information | activity only in adult worms | Schistosoma japonicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | additional information | - |
- |
Schistosoma mansoni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | Hemoglobin + H2O | - |
Schistosoma japonicum | ? | - |
? | |
| 3.4.22.34 | Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma japonicum | ? | - |
? | |
| 3.4.22.34 | Hemoglobin + H2O | the recombinant beta-galactosidase fusion enzyme shows hemoglobinase activity | Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | additional information | the enzyme strictly requires Asn at P1, and prefers Ala, Thr, Val, Asn in descending order at P2, and Thr, Ala, Val, and Ile at P3, overview | Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.34 | Z-Ala-Pro-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Pro-Asn + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.34 | Z-Ala-Ser-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ser-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | ? | x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| 3.4.22.34 | ? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| 3.4.22.34 | ? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
| 3.4.22.34 | More | structure analysis, overview | Schistosoma mansoni |
| 3.4.22.34 | More | structure analysis, overview | Schistosoma japonicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | Antigen Sj32 | - |
Schistosoma japonicum |
| 3.4.22.34 | Antigen SM32 | - |
Schistosoma mansoni |
| 3.4.22.34 | C197 legumain | - |
Schistosoma mansoni |
| 3.4.22.34 | c197 Sm32 | - |
Schistosoma mansoni |
| 3.4.22.34 | cysteine protease 1 | - |
Homo sapiens |
| 3.4.22.34 | hemoglobinase | - |
Schistosoma mansoni |
| 3.4.22.34 | hemoglobinase | - |
Schistosoma japonicum |
| 3.4.22.34 | LGMN | - |
Homo sapiens |
| 3.4.22.34 | More | the enzyme belongs to the C13 peptidase family | Homo sapiens |
| 3.4.22.34 | More | the enzyme belongs to the C13 peptidase family | Schistosoma mansoni |
| 3.4.22.34 | More | the enzyme belongs to the C13 peptidase family | Schistosoma japonicum |
| 3.4.22.34 | N197 legumain | - |
Schistosoma mansoni |
| 3.4.22.34 | schistosome legumain | - |
Schistosoma mansoni |
| 3.4.22.34 | schistosome legumain | - |
Schistosoma japonicum |
| 3.4.22.34 | Sj32 | - |
Schistosoma japonicum |
| 3.4.22.34 | Sm32 | - |
Schistosoma mansoni |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 37 | 45 | - |
Schistosoma mansoni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 6.8 | - |
- |
Schistosoma mansoni |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.34 | 5.5 | 6.8 | recombinant enzyme | Schistosoma mansoni |
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