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Literature summary extracted from

  • Chen, J.; Dando, P.M.; Barrett, A.J.
    Mammalian legumain (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1302-1305.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.34 additional information the enzyme requires activation by a thiol compound Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 DNA sequence and structure determination and analysis, expression in mammalian cells Mus musculus
3.4.22.34 gene PRSC1, DNA and amino acid sequence determination and analysis, localization on chromosome 14q32.1, expression in insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.34
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.34 additional information construction of null-legumain mice Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 cystatin from chicken egg white Sus scrofa
3.4.22.34 cystatin C human Sus scrofa
3.4.22.34 iodoacetamide
-
 Sus scrofa
3.4.22.34 iodoacetate
-
 Sus scrofa
3.4.22.34 additional information no inhibition by E64 and leupeptin Sus scrofa
3.4.22.34 N-ethylmaleimide
-
 Sus scrofa
3.4.22.34 N-Phenylmaleimide strong inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.34 lysosome
-
 Mus musculus 5764
-
3.4.22.34 lysosome
-
 Homo sapiens 5764
-
3.4.22.34 lysosome
-
 Sus scrofa 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 31000
-
 x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme Homo sapiens
3.4.22.34 49000
-
 x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 additional information Homo sapiens the inhibition of osteoclast formation and bone resorption is a nonenzymatic function of prolegumain ?
-
 ?
3.4.22.34 neurotensin + H2O Sus scrofa specific cleavage ?
-
 ?
3.4.22.34 progelatinase A + H2O Homo sapiens activation gelatinase A + gelatinase A propeptide
-
 ?
3.4.22.34 Vasoactive intestinal peptide + H2O Sus scrofa specific cleavage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens
-
-
-
3.4.22.34 Mus musculus
-
-
-
3.4.22.34 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.34 glycoprotein
-
 Homo sapiens
3.4.22.34 glycoprotein N-glycosylation, deglycosylation by N-glycosidase F Sus scrofa
3.4.22.34 proteolytic modification autoactivation/maturation by cleavage at Asn323 Homo sapiens
3.4.22.34 proteolytic modification autoactivation/maturation by cleavage at Asn323 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 native enzyme from kidney, overview Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.34 hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds the enzyme contains essential catalytic His and Cys residues Mus musculus
a
3.4.22.34 hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds the enzyme contains essential catalytic His and Cys residues Homo sapiens
a
3.4.22.34 hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds the enzyme contains essential catalytic His and Cys residues Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 kidney
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.34 4°C, purified native enzyme from kidney, pH 5.8, little loss of activity over several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 Boc-Asn-4-nitrophenyl ester + H2O
-
 Homo sapiens Boc-Asn + 4-nitrophenol
-
 ?
3.4.22.34 additional information the inhibition of osteoclast formation and bone resorption is a nonenzymatic function of prolegumain Homo sapiens ?
-
 ?
3.4.22.34 additional information the enzyme acts strictly as asparaginyl endopeptidase, substrate specificity, overview Sus scrofa ?
-
 ?
3.4.22.34 neurotensin + H2O specific cleavage Sus scrofa ?
-
 ?
3.4.22.34 progelatinase A + H2O
-
 Homo sapiens gelatinase A + gelatinase A propeptide
-
 ?
3.4.22.34 progelatinase A + H2O activation Homo sapiens gelatinase A + gelatinase A propeptide
-
 ?
3.4.22.34 Vasoactive intestinal peptide + H2O specific cleavage Sus scrofa ?
-
 ?
3.4.22.34 Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 49000, proenzyme, x * 31000, mature, deglycosylated enzyme Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 Asparaginyl endopeptidase
-
 Mus musculus
3.4.22.34 Asparaginyl endopeptidase
-
 Homo sapiens
3.4.22.34 Asparaginyl endopeptidase
-
 Sus scrofa
3.4.22.34 More the enzyme belongs to the C13 peptidase family Mus musculus
3.4.22.34 More the enzyme belongs to the C13 peptidase family Homo sapiens
3.4.22.34 More the enzyme belongs to the C13 peptidase family Sus scrofa
3.4.22.34 osteoclast inhibitory peptide 2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.8
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.34 3 6 stable, irreversible denaturation at higher pH Sus scrofa