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Literature summary extracted from

  • Joshua-Tor, L.; Johnston, S.A.
    Bleomycin hydrolase (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1197-1201.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.40 additional information binding to nucleic acids, preferably single-stranded DNA or RNA, is required for enzyme activity Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.40 DNA sequence determination Rattus norvegicus
3.4.22.40 DNA sequence determination Oryctolagus cuniculus
3.4.22.40 DNA sequence determination and analysis, chromosome localization at 19q11.2 Homo sapiens
3.4.22.40 gene YCP1 or BLH1, DNA sequence determination Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.40 additional information mutant pre3-2 is inactive with substrate Z-Leu-Leu-Glu-NH-4-nitroanilide, but can be complemented b ythe wild-type gene Saccharomyces cerevisiae
3.4.22.40 V443I the naturally occurring polymorphism of the gene encoding the enzyme is associated to sporadic Alzheimer's disease and to the amyloid precursor protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.40 4-hydroxymeruribenzoate
-
Homo sapiens
3.4.22.40 4-hydroxymeruribenzoate
-
Oryctolagus cuniculus
3.4.22.40 4-hydroxymeruribenzoate
-
Rattus norvegicus
3.4.22.40 4-hydroxymeruribenzoate
-
Streptomyces verticillus
3.4.22.40 E64
-
Homo sapiens
3.4.22.40 E64
-
Oryctolagus cuniculus
3.4.22.40 E64
-
Rattus norvegicus
3.4.22.40 E64
-
Streptomyces verticillus
3.4.22.40 iodoacetate
-
Homo sapiens
3.4.22.40 iodoacetate
-
Oryctolagus cuniculus
3.4.22.40 iodoacetate
-
Rattus norvegicus
3.4.22.40 iodoacetate
-
Streptomyces verticillus
3.4.22.40 leupeptin weak inhibition Rattus norvegicus
3.4.22.40 additional information no inhibition by leupeptin Homo sapiens
3.4.22.40 additional information no inhibition by cystatin A and cystatin C Rattus norvegicus
3.4.22.40 additional information no inhibition by leupeptin Streptomyces verticillus
3.4.22.40 NEM
-
Homo sapiens
3.4.22.40 NEM
-
Oryctolagus cuniculus
3.4.22.40 NEM
-
Rattus norvegicus
3.4.22.40 NEM
-
Streptomyces verticillus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.40 additional information a self-compartmentalizing enzyme Homo sapiens
-
-
3.4.22.40 additional information a self-compartmentalizing enzyme Rattus norvegicus
-
-
3.4.22.40 additional information a self-compartmentalizing enzyme Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.40 additional information glycometalloenzyme Streptomyces verticillus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.40 300000
-
about Homo sapiens
3.4.22.40 300000
-
about Rattus norvegicus
3.4.22.40 300000
-
about Oryctolagus cuniculus
3.4.22.40 300000
-
about Streptomyces verticillus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.40 bleomycin + H2O Rattus norvegicus detoxification reaction ?
-
?
3.4.22.40 bleomycin + H2O Oryctolagus cuniculus detoxification reaction ?
-
?
3.4.22.40 bleomycin + H2O Homo sapiens detoxification reaction, high enzyme expression level leads to resistance against the drug ?
-
?
3.4.22.40 additional information Saccharomyces cerevisiae the enzyme expression is regulated by galactose and Gal4p,expression pattrern ?
-
?
3.4.22.40 Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O Saccharomyces cerevisiae no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p Z-Leu-Leu-Glu + 4-nitroaniline
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.40 Homo sapiens
-
-
-
3.4.22.40 Oryctolagus cuniculus
-
-
-
3.4.22.40 Rattus norvegicus
-
-
-
3.4.22.40 Saccharomyces cerevisiae
-
gene YCP1 or BLH1
-
3.4.22.40 Streptomyces verticillus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.40 glycoprotein glycometalloenzyme Streptomyces verticillus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.40 brain
-
Homo sapiens
-
3.4.22.40 Burkitt's lymphoma cell high expression level Homo sapiens
-
3.4.22.40 leukemia cell high expression level Homo sapiens
-
3.4.22.40 additional information no expression in Hodgkin's lymphoma cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.40 bleomycin + H2O
-
Rattus norvegicus ?
-
?
3.4.22.40 bleomycin + H2O
-
Oryctolagus cuniculus ?
-
?
3.4.22.40 bleomycin + H2O detoxification reaction Rattus norvegicus ?
-
?
3.4.22.40 bleomycin + H2O detoxification reaction Oryctolagus cuniculus ?
-
?
3.4.22.40 bleomycin + H2O detoxification reaction, high enzyme expression level leads to resistance against the drug Homo sapiens ?
-
?
3.4.22.40 bleomycin A2 + H2O preferred to bleomycin B Homo sapiens ?
-
?
3.4.22.40 bleomycin B2 + H2O
-
Homo sapiens ?
-
?
3.4.22.40 additional information the enzyme expression is regulated by galactose and Gal4p,expression pattrern Saccharomyces cerevisiae ?
-
?
3.4.22.40 additional information the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview Homo sapiens ?
-
?
3.4.22.40 additional information the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview Rattus norvegicus ?
-
?
3.4.22.40 additional information the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview Oryctolagus cuniculus ?
-
?
3.4.22.40 Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p Saccharomyces cerevisiae Z-Leu-Leu-Glu + 4-nitroaniline
-
?

Subunits

EC Number Subunits Comment Organism
3.4.22.40 hexamer homohexamer, three-dimensional structure Homo sapiens
3.4.22.40 hexamer homohexamer, three-dimensional structure Rattus norvegicus
3.4.22.40 hexamer homohexamer, three-dimensional structure Oryctolagus cuniculus
3.4.22.40 hexamer homohexamer, three-dimensional structure Streptomyces verticillus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.40 BH
-
Homo sapiens
3.4.22.40 BH
-
Rattus norvegicus
3.4.22.40 BH
-
Saccharomyces cerevisiae
3.4.22.40 BH
-
Oryctolagus cuniculus
3.4.22.40 BH
-
Streptomyces verticillus
3.4.22.40 Gal6p
-
Saccharomyces cerevisiae
3.4.22.40 More the enzyme belongs to the C1B peptidase family Homo sapiens
3.4.22.40 More the enzyme belongs to the C1B peptidase family Rattus norvegicus
3.4.22.40 More the enzyme belongs to the C1B peptidase family Saccharomyces cerevisiae
3.4.22.40 More the enzyme belongs to the C1B peptidase family Oryctolagus cuniculus
3.4.22.40 yeast cysteine protease
-
Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.40 6.5 7
-
Streptomyces verticillus
3.4.22.40 7 7.5
-
Oryctolagus cuniculus
3.4.22.40 7.2
-
-
Homo sapiens
3.4.22.40 7.5
-
-
Saccharomyces cerevisiae