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Literature summary extracted from

  • Woessner, J.F.
    Nepenthesin (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 1, 85-86.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.12 Diazoacetyl-DL-norleucine methyl ester i.e. DAN Nepenthes distillatoria
3.4.23.12 N-(diazoacetyl)-N-(2,4-dinitrophenyl)ethylenediamine
-
 Nepenthes distillatoria
3.4.23.12 pepstatin
-
 Nepenthes distillatoria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.12 extracellular the enzyme is secreted to the pitcher fluid Nepenthes distillatoria
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.12 45000
-
 1 * 45000 Nepenthes distillatoria

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.12 Nepenthes distillatoria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.12 by gel filtration, ion exchange chromatography, and pepstatin affinity chromatography, native enzyme to homogeneity Nepenthes distillatoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.12 pitcher secretory gland inside the pitcher Nepenthes distillatoria
-
3.4.23.12 pitcher secretion
-
 Nepenthes distillatoria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.12 casein + H2O
-
 Nepenthes distillatoria ?
-
 ?
3.4.23.12 Egg albumin + H2O
-
 Nepenthes distillatoria ?
-
 ?
3.4.23.12 Fibrin + H2O
-
 Nepenthes distillatoria ?
-
 ?
3.4.23.12 FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O substrate is the insulin B chain Nepenthes distillatoria FVNQHL + CGSHLVE + Ala-Leu + Tyr + LVCGERGF + FYTPKA
-
 ?
3.4.23.12 additional information cleavage site specificity with preference for the carboxy and amino sides of Asp, cleavage of the carboxy side of Ala, Leu, Ser, Thr, and Tyr also occurs, and cleavage on the amino side of Ala, Phe, Thr, Tyr, and Lys Nepenthes distillatoria ?
-
 ?
3.4.23.12 Ribonuclease + H2O
-
 Nepenthes distillatoria ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.12 monomer 1 * 45000 Nepenthes distillatoria

Synonyms

EC Number Synonyms Comment Organism
3.4.23.12 More the enzyme belongs to the A1 peptidase family Nepenthes distillatoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.12 2 3
-
 Nepenthes distillatoria