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Literature summary extracted from
- Cathepsin V (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd Ed., 1107-1109.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.22.43 | diagnostics | the enzyme is a potential marker for certain colon tumors and breast cancer | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.43 | from cDNA libraries, the gene maps to chromosome 9q21, DNA and amino acid sequence determination and analysis, genomic organization, expression in insect cells using the baculovirus system, in Pichia pastoris, and as GST-tagged enzyme with low activity in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.43 | calpeptin | - |
Homo sapiens |  |
| 3.4.22.43 | cystatins | - |
Homo sapiens | |
| 3.4.22.43 | E-64 | - |
Homo sapiens | |
| 3.4.22.43 | leupeptin | - |
Homo sapiens | |
| 3.4.22.43 | peptidl vinyl sulfones | - |
Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.43 | extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.43 | 23999 | - |
2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris | Homo sapiens |
| 3.4.22.43 | 31000 | - |
2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris | Homo sapiens |
| 3.4.22.43 | 52000 | - |
recombinant GST-tagged enzyme | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.43 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.43 | glycoprotein | N-glycosylation of extracellular proenzyme and mature enzyme | Homo sapiens |
| 3.4.22.43 | proteolytic modification | autoactivation of the inactive extracellular zymogen at pH 4.0 and 37°C to the active mature protein | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.43 | recombinant enzyme from Pichia pastoris to homogeneity, activated mature native enzyme by hyrophobic interaction chromatography, recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.43 | cornea | epithelium | Homo sapiens | - |
| 3.4.22.43 | epithelium | thymic and corneal | Homo sapiens | - |
| 3.4.22.43 | additional information | selective, tissue-specific expression | Homo sapiens | - |
| 3.4.22.43 | testis | - |
Homo sapiens | - |
| 3.4.22.43 | thymus | epithelium | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.43 | Albumin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.22.43 | MHC class II-associated invariant chain + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.22.43 | additional information | the enzyme prefers hydrophobic residues in its S2-binding pocket, the substrate specificity is similar to cathepsin L, no cleavage of the triple-helical region of native collagen | Homo sapiens | ? | - |
? | |
| 3.4.22.43 | Type I collagen + H2O | cleavage of telopeptide region | Homo sapiens | ? | - |
? | |
| 3.4.22.43 | Z-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.43 | Z-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Z-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.43 | dimer | 2 * 23999, amino acid sequence calculation of the mature enzyme, 2 * 31000, recombinant mature enzyme from insect cells and Pichia pastoris, 2 * 38000-45000, recombinant proenzyme from Pichi pastoris | Homo sapiens |
| 3.4.22.43 | More | three-dimensional two-domain structure analysis at 1.6 A resolution, overview | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.43 | cathepsin L2 | - |
Homo sapiens |
| 3.4.22.43 | cathepsin U | - |
Homo sapiens |
| 3.4.22.43 | More | the enzyme belongs to the C1A peptidase family | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.43 | 5.5 | 6 | - |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.43 | additional information | - |
bell-shaped pH profile | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.43 | additional information | - |
rapid loss of activity at neutral pH | Homo sapiens |
| 3.4.22.43 | 4.5 | 5.5 | very stable | Homo sapiens |
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