EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.57 | synthesis | enzymatic method of L-tryptophan production. The enzyme is useful for the manufacturing process because it is not inhibited by high levels of product | Cutaneotrichosporon cutaneum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.57 | 2,2'-dipyridyl | - |
Cutaneotrichosporon cutaneum | |
3.5.1.57 | additional information | no inhibition by high levels of the product L-tryptophan | Cutaneotrichosporon cutaneum | |
3.5.1.57 | NEM | - |
Cutaneotrichosporon cutaneum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.57 | cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.57 | Co2+ | 25% of the activation with Mn2+ | Cutaneotrichosporon cutaneum | |
3.5.1.57 | Mn2+ | 2.5 mM required for full activity | Cutaneotrichosporon cutaneum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
3.5.1.57 | 270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.57 | Cutaneotrichosporon cutaneum | - |
IFO 0173 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.57 | L-alaninamide + H2O | hydrolyzed 14% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-alanine + NH3 | - |
? | |
3.5.1.57 | L-asparagine + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-aspartate + NH3 | - |
? | |
3.5.1.57 | L-citrulline + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | ? | - |
? | |
3.5.1.57 | L-glutamine + H2O | hydrolyzed 18% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-glutamate + NH3 | - |
? | |
3.5.1.57 | L-leucinamide + H2O | hydrolyzed 26% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-leucine + NH3 | - |
? | |
3.5.1.57 | L-methioninamide + H2O | hydrolyzed 14% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-methionine + NH3 | - |
? | |
3.5.1.57 | L-phenylalaninamide + H2O | hydrolyzed 47% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-phenylalanine + NH3 | - |
? | |
3.5.1.57 | L-serinamide + H2O | hydrolyzed 7% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-serine + NH3 | - |
? | |
3.5.1.57 | L-tryptophanamide + H2O | - |
Cutaneotrichosporon cutaneum | L-tryptophan + NH3 | - |
? | |
3.5.1.57 | L-tyrosinamide + H2O | hydrolyzed 22% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-tyrosine + NH3 | - |
? | |
3.5.1.57 | L-valinamide + H2O | hydrolyzed 15% as rapidly as L-tryptophanamide | Cutaneotrichosporon cutaneum | L-valine + NH3 | - |
? | |
3.5.1.57 | additional information | high affinity towards peptides having a L-Trp residue at the N-terminal moiety | Cutaneotrichosporon cutaneum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.57 | tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 40 | 45 | - |
Cutaneotrichosporon cutaneum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 55 | - |
10 min, stable | Cutaneotrichosporon cutaneum |
3.5.1.57 | 60 | - |
rapid inactivation | Cutaneotrichosporon cutaneum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 9 | 9.5 | - |
Cutaneotrichosporon cutaneum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 7.5 | 8.5 | room temperature, 20 h, no appreciable loss of activity | Cutaneotrichosporon cutaneum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.5.1.57 | Cutaneotrichosporon cutaneum | isoelectrofocusing | - |
4.7 |