Literature summary extracted from

Jozwiak, J.; Komar, A.; Jankowska, E.; Martirosian, G.
Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors (2005), FEMS Immunol. Med. Microbiol., 45, 137-142.

Application

EC Number	Application	Comment	Organism
3.4.22.8	medicine	influence of protease inhibitors aprotinin, phenylmethylsulfonyl fluoride, L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone, and chymostatin on toxicity of Clostridium histolyticum supernatant towards HeLa cells. Aprotinin has no effect, while the other inhibitors potentiate the cytotoxic activity of Clostridium histolyticum probybly by hindering the natural defense of cells. In addition, phenylmethylsulfonyl fluoride and L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone block clostripain enzymic activity	Hathewaya histolytica

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.22.8	L-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone	complete inhibition	Hathewaya histolytica
3.4.22.8	phenylmethylsulfonyl fluoride	complete inhibition	Hathewaya histolytica

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.8	Hathewaya histolytica	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

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Release 2023.1 (January 2023)