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Literature summary extracted from

  • Amako, K.; Fujita, K.; Shimohata, T.A.; Hasegawa, E.; Kishimoto, R.; Goda, K.
    NAD(+)-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae (2006), FEBS Lett., 580, 6428-6434.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.116 gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.116 additional information ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.116 D-arabinose substrate inhibition of Ara2p at concentrations above 5 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.116 0.78
-
 D-arabinose pH 8.2, 25°C, recombinant His-tagged Ara2p Saccharomyces cerevisiae
1.1.1.116 4.1
-
 NAD+ pH 8.2, 25°C, recombinant His-tagged Ara2p Saccharomyces cerevisiae
1.1.3.37 0.78
-
 D-arabinose
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.116 42000
-
 recombinant Ara2p, gel filtration Saccharomyces cerevisiae
1.1.1.116 42400
-
 1 * 42400, recombinant Ara2p, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.116 D-arabinose + NAD+ Saccharomyces cerevisiae the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+ Saccharomyces cerevisiae YPH250 the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose D-arabinono-1,4-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.116 Saccharomyces cerevisiae Q04212 ARA2; gene YMR041c or ARA2, isozyme Ara2p
-
1.1.1.116 Saccharomyces cerevisiae YPH250 Q04212 ARA2; gene YMR041c or ARA2, isozyme Ara2p
-
1.1.3.37 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.116 recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.116 0.00071
-
 NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract Saccharomyces cerevisiae
1.1.1.116 1.8
-
 purified recombinant His-tagged ARA2p Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.116 D-arabinose + NAD+ the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+ preferred sugar substrate Saccharomyces cerevisiae D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+ the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose Saccharomyces cerevisiae YPH250 D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 D-arabinose + NAD+ preferred sugar substrate Saccharomyces cerevisiae YPH250 D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.116 L-fucose + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.116 L-fucose + NAD+
-
 Saccharomyces cerevisiae YPH250 ?
-
 ?
1.1.1.116 L-galactose + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.116 L-galactose + NAD+
-
 Saccharomyces cerevisiae YPH250 ?
-
 ?
1.1.1.116 L-xylose + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.116 additional information ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose Saccharomyces cerevisiae ?
-
 ?
1.1.1.116 additional information ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose Saccharomyces cerevisiae YPH250 ?
-
 ?
1.1.3.37 D-arabinose + NAD+
-
 Saccharomyces cerevisiae erythroascorbic acid + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.116 monomer 1 * 42400, recombinant Ara2p, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.116 ARA
-
 Saccharomyces cerevisiae
1.1.1.116 Ara2p
-
 Saccharomyces cerevisiae
1.1.1.116 NAD(+)-specific D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae
1.1.3.37 Ara2p
-
 Saccharomyces cerevisiae
1.1.3.37 NAD+ - specific D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.116 25
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.116 8.2
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.116 6.5 9 20% of maximal activity at pH 6.5 and pH 9.0, pH profile Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.116 NAD+ Ara2p is specific for NAD+, while Ara1p utilizes NADP+ Saccharomyces cerevisiae