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Literature summary extracted from

  • Kataoka, S.; Arakawa, T.; Hori, S.; Katayama, Y.; Hara, Y.; Matsushita, Y.; Nakayama, H.; Yohda, M.; Nyunoya, H.; Dohmae, N.; Maeda, M.; Odaka, M.
    Functional expression of thiocyanate hydrolase is promoted by its activator protein, P15K (2006), FEBS Lett., 580, 4667-4672.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.8 additional information expression of thiocyanate hydrolase is promoted by its activator protein P15K Thiobacillus thioparus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.8 expression of the alpha, beta, and gamma subunits in Escherichia coli, the subunits assemble to an apo-heterododecamer (alphabetagamma)4 like the wild-type enzyme exhibiting no catalytic activity due to lack of bound Co2+ irrespective of the cobalt concentration in the medium, coepression of enzyme with activator protein P15K leads to a functional Co2+-bound enzyme which exhibits 78% of native enzyme activity, optimization of conditions, overview Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.8 Co2+ cobalt-containing metalloenzyme, 0.86 Co2+ per enzyme trimer, binding to the gamma-subunit requires the presence of activator protein P15K mediating the Co2+ incorporation, overview Thiobacillus thioparus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.8 140000
-
 native and apo-enzyme, gel filtration Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.8 thiocyanate + H2O Thiobacillus thioparus
-
 carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O Thiobacillus thioparus THI115
-
 carbonyl sulfide + NH3 + OH-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Thiobacillus thioparus
-
-
-
3.5.5.8 Thiobacillus thioparus THI115
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.8 native enzyme from strain THI115, and recombinant enzyme from Echerichia coli strain BL21(DE3) by three steps of butyl-resin chromatography, hydroxyapatite and ion exchange chromatography, or by 4 steps of butyl-resin chromatography and gel filtration Thiobacillus thioparus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.8 32
-
 purified native enzyme Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + OH-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.8 dodecamer heterododecamer (alphabetagamma)4 of heterotrimers Thiobacillus thioparus

Synonyms

EC Number Synonyms Comment Organism
3.5.5.8 SCNase
-
 Thiobacillus thioparus