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Literature summary extracted from

  • Du, X.; Tove, S.; Kast-Hutcheson, K.; Grunden, A.M.
    Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase by analysis of targeted mutants (2005), FEBS Lett., 579, 6140-6146.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.9 D209A less than 0.1% enzymic activity, contains 0.7 Co per subunit, maximal activity with 0.5 mM Co2+, less than 20% residual activity with 10 mM Co2+ Pyrococcus furiosus
3.4.13.9 E313L protein is highly misfolded, remains aggregated and recalcitrant during purification Pyrococcus furiosus
3.4.13.9 E327L no enzymic activity, contains 0.03 Co per sunbunit Pyrococcus furiosus
3.4.13.9 H284A less than 0.1% enzymic activity, protein is unfolded Pyrococcus furiosus
3.4.13.9 H284L less than 0.1% enzymic activity, contains 0.28 Co per subunit. Inhibitory effect of high Co2+-concentration is less pronounced than in wild-type Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.9 Co2+ one Co-bound dinuclear metal cluster per monomer, Co1 is the tight binding, Co2 the loose binding metal center. Wild-type exhibits maximal activity in presence of 0.5 mM Co2+, less than 20% residual activity with 10 mM Co2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.9 42000
-
 x * 42000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Pyrococcus furiosus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.9 0.67
-
 mutant H284A, pH 7.0, 100°C Pyrococcus furiosus
3.4.13.9 0.73
-
 mutant H284L, pH 7.0, 100°C Pyrococcus furiosus
3.4.13.9 1.02
-
 mutant D209A, pH 7.0, 100°C Pyrococcus furiosus
3.4.13.9 1388
-
 wild-type, pH 7.0, 100°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 L-Met-L-Pro
-
 Pyrococcus furiosus L-Met + L-Pro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.9 ? x * 42000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.9 100
-
-
 Pyrococcus furiosus