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Literature summary extracted from

  • Mizuno, K.; Okuda, A.; Kato, M.; Yoneyama, N.; Tanaka, H.; Ashihara, H.; Fujimura, T.
    Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee (Coffea arabica L.) (2003), FEBS Lett., 534, 75-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.159 DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein Coffea arabica
2.1.1.160 DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein Coffea arabica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.159 0.031
-
 paraxanthine pH 8.5, 37°C, recombinant CCS1 Coffea arabica
2.1.1.159 0.075
-
 7-methylxanthine pH 8.5, 37°C, recombinant Trx-fusion CCS1 Coffea arabica
2.1.1.159 0.126
-
 7-methylxanthine pH 8.5, 37°C, recombinant CCS1 Coffea arabica
2.1.1.160 0.157
-
 Theobromine pH 8.5, 37°C, recombinant CCS1 Coffea arabica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.159 additional information Coffea arabica bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica third step in caffeine biosynthesis pathway from xanthosine S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 additional information Coffea arabica bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica last step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.159 Coffea arabica Q8H0D3 bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160; isozyme CtCS6 is a binfunctional enzyme
-
2.1.1.160 Coffea arabica Q8H0D3 bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160; isozyme CtCS6 is a bifunctional enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.160 endosperm developing Coffea arabica
-
2.1.1.160 flower bud
-
 Coffea arabica
-
2.1.1.160 leaf young Coffea arabica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.159 4.59
-
 recombinant CCS1 Coffea arabica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.159 additional information bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
2.1.1.159 additional information substrate specificity, bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine third step in caffeine biosynthesis pathway from xanthosine Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.159 S-adenosyl-L-methionine + paraxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 additional information bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
2.1.1.160 additional information substrate specificity, bifunctional enzyme performing 3-N-methyltransferase activity, EC 2.1.1.159, and 1-N-methyltransferase activity, EC 2.1.1.160, overview Coffea arabica ?
-
 ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine last step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.159 3-N-methyltransferase
-
 Coffea arabica
2.1.1.159 caffeine synthase 1
-
 Coffea arabica
2.1.1.159 CCS1
-
 Coffea arabica
2.1.1.159 coffee caffeine synthase 1
-
 Coffea arabica
2.1.1.159 CtCS6
-
 Coffea arabica
2.1.1.160 1-N-methyltransferase
-
 Coffea arabica
2.1.1.160 caffeine synthase 1
-
 Coffea arabica
2.1.1.160 CCS1
-
 Coffea arabica
2.1.1.160 coffee caffeine synthase 1
-
 Coffea arabica
2.1.1.160 CtCS6
-
 Coffea arabica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.159 37
-
 assay at Coffea arabica
2.1.1.160 37
-
 assay at Coffea arabica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.159 8.5
-
 assay at Coffea arabica
2.1.1.160 8.5
-
 assay at Coffea arabica