BRENDA - Enzyme Database

5-Methyldeoxycytidine monophosphate deaminase and 5-methylcytidyl-DNA deaminase activities are present in human mature sperm cells

Jost, J.P.; Thiry, S.; Siegmann, M.; FEBS Lett. 519, 128-134 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
additional information
no activation by deoxynucleotide triphosphates
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
additional information
no inhibition by deoxynucleotide triphosphates
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetics
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
additional information
no metals required
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
Homo sapiens
deamination pathway, overview
dTMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Homo sapiens
-
dUMP + NH3
-
-
?
3.5.4.12
additional information
Homo sapiens
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
native enzyme from sperm cells by ion exchange chromatography and preparative SDS-PAGE
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
sperm cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
668584
Homo sapiens
dTMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
deamination pathway, overview
668584
Homo sapiens
dTMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
668584
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
668584
Homo sapiens
?
-
-
-
-
3.5.4.12
additional information
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, substrate specificity, overview
668584
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
additional information
no activation by deoxynucleotide triphosphates
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
additional information
no inhibition by deoxynucleotide triphosphates
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
additional information
-
additional information
kinetics
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
additional information
no metals required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
Homo sapiens
deamination pathway, overview
dTMP + NH3
-
-
?
3.5.4.12
dCMP + H2O
Homo sapiens
-
dUMP + NH3
-
-
?
3.5.4.12
additional information
Homo sapiens
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
native enzyme from sperm cells by ion exchange chromatography and preparative SDS-PAGE
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.12
sperm cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
5-methyl-dCMP + H2O
-
668584
Homo sapiens
dTMP + NH3
-
-
-
?
3.5.4.12
5-methyl-dCMP + H2O
deamination pathway, overview
668584
Homo sapiens
dTMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
668584
Homo sapiens
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
668584
Homo sapiens
?
-
-
-
-
3.5.4.12
additional information
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, substrate specificity, overview
668584
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.12
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7.5
-
assay at
Homo sapiens