BRENDA - Enzyme Database

Proteins involved in lipid translocation in eukaryotic cells

Devaux, P.F.; Lopez-MOntero, I.; Bryde, S.; Chem. Phys. Lipids 141, 119-132 (2006)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.6.2.1
additional information
simultaneous inactivation of Dnf2p and Dnf1p abolishes the ATP-dependent influx of phosphatidylserine and phosphatidylethanolamine into the cell
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
7.6.2.1
the enzyme is very instable and rapidly degenerated during purification
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
Ca2+
-
Homo sapiens
7.6.2.1
NEM
-
Homo sapiens
7.6.2.1
vanadate
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
cell membrane
-
Bos taurus
-
-
7.6.2.1
cell membrane
-
Homo sapiens
-
-
7.6.2.1
cell membrane
-
Leishmania infantum
-
-
7.6.2.1
cell membrane
-
Saccharomyces cerevisiae
-
-
7.6.2.1
endoplasmic reticulum
Neo1p
Saccharomyces cerevisiae
5783
-
7.6.2.1
endoplasmic reticulum membrane
-
Homo sapiens
5789
-
7.6.2.1
endoplasmic reticulum membrane
-
Rattus norvegicus
5789
-
7.6.2.1
endosome
Neo1p
Saccharomyces cerevisiae
5768
-
7.6.2.1
Golgi membrane
Drs2p and Dnf3p
Saccharomyces cerevisiae
139
-
7.6.2.1
additional information
chromaffine granules
Bos taurus
-
-
7.6.2.1
plasma membrane
Dnf1p and Dnf2p
Saccharomyces cerevisiae
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.1
Ca2+
scramblase activity is dependent on Ca2+
Homo sapiens
7.6.2.1
Mg2+
dependent on
Bos taurus
7.6.2.1
Mg2+
dependent on
Leishmania infantum
7.6.2.1
Mg2+
dependent on
Saccharomyces cerevisiae
7.6.2.1
Mg2+
dependent on
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.6.2.1
37000
-
x * 37000, scramblase
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Homo sapiens
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Saccharomyces cerevisiae
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Bos taurus
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Leishmania infantum
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Homo sapiens
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Saccharomyces cerevisiae
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Bos taurus
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Leishmania infantum
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
additional information
Homo sapiens
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Bos taurus
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Leishmania infantum
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Saccharomyces cerevisiae
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
?
-
-
-
7.6.2.1
phospholipid/in
Homo sapiens
unspecific bidirectional transmembrane transport of phospholipids
phospholipid/out
-
-
r
7.6.2.1
phospholipid/in
Rattus norvegicus
unspecific bidirectional transmembrane transport of phospholipids
phospholipid/out
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.1
Bos taurus
-
-
-
7.6.2.1
Homo sapiens
-
-
-
7.6.2.1
Leishmania infantum
-
-
-
7.6.2.1
Rattus norvegicus
-
-
-
7.6.2.1
Saccharomyces cerevisiae
-
five P-type ATPases involved inlipid transport
-
Purification (Commentary)
EC Number
Commentary
Organism
7.6.2.1
ATP8A1 from chromaffin granules
Bos taurus
7.6.2.1
partially from erythrocytes
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.1
blood platelet
-
Homo sapiens
-
7.6.2.1
endothelial cell
-
Homo sapiens
-
7.6.2.1
erythrocyte
-
Homo sapiens
-
7.6.2.1
fibroblast
-
Homo sapiens
-
7.6.2.1
liver
-
Rattus norvegicus
-
7.6.2.1
osteoblast
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Homo sapiens
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Saccharomyces cerevisiae
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Bos taurus
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Leishmania infantum
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Homo sapiens
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Saccharomyces cerevisiae
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Bos taurus
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Leishmania infantum
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane, and from the lumenal side of granules to the external side
668247
Bos taurus
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Bos taurus
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Leishmania infantum
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
668247
Saccharomyces cerevisiae
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Saccharomyces cerevisiae
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Bos taurus
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Leishmania infantum
?
-
-
-
-
7.6.2.1
additional information
the enzyme is independent of ATP or energy supply
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme is independent of ATP or energy supply
668247
Rattus norvegicus
?
-
-
-
-
7.6.2.1
phospholipid/in
unspecific bidirectional transmembrane transport of phospholipids
668247
Homo sapiens
phospholipid/out
-
-
-
r
7.6.2.1
phospholipid/in
unspecific bidirectional transmembrane transport of phospholipids
668247
Rattus norvegicus
phospholipid/out
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
7.6.2.1
?
x * 37000, scramblase
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.6.2.1
additional information
simultaneous inactivation of Dnf2p and Dnf1p abolishes the ATP-dependent influx of phosphatidylserine and phosphatidylethanolamine into the cell
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
7.6.2.1
the enzyme is very instable and rapidly degenerated during purification
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
Ca2+
-
Homo sapiens
7.6.2.1
NEM
-
Homo sapiens
7.6.2.1
vanadate
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
cell membrane
-
Bos taurus
-
-
7.6.2.1
cell membrane
-
Homo sapiens
-
-
7.6.2.1
cell membrane
-
Leishmania infantum
-
-
7.6.2.1
cell membrane
-
Saccharomyces cerevisiae
-
-
7.6.2.1
endoplasmic reticulum
Neo1p
Saccharomyces cerevisiae
5783
-
7.6.2.1
endoplasmic reticulum membrane
-
Homo sapiens
5789
-
7.6.2.1
endoplasmic reticulum membrane
-
Rattus norvegicus
5789
-
7.6.2.1
endosome
Neo1p
Saccharomyces cerevisiae
5768
-
7.6.2.1
Golgi membrane
Drs2p and Dnf3p
Saccharomyces cerevisiae
139
-
7.6.2.1
additional information
chromaffine granules
Bos taurus
-
-
7.6.2.1
plasma membrane
Dnf1p and Dnf2p
Saccharomyces cerevisiae
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.6.2.1
Ca2+
scramblase activity is dependent on Ca2+
Homo sapiens
7.6.2.1
Mg2+
dependent on
Bos taurus
7.6.2.1
Mg2+
dependent on
Leishmania infantum
7.6.2.1
Mg2+
dependent on
Saccharomyces cerevisiae
7.6.2.1
Mg2+
dependent on
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.6.2.1
37000
-
x * 37000, scramblase
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Homo sapiens
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Saccharomyces cerevisiae
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Bos taurus
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
Leishmania infantum
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylethanolamine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Homo sapiens
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Saccharomyces cerevisiae
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Bos taurus
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
Leishmania infantum
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
ADP + phosphate + phosphatidylserine/in
-
-
?
7.6.2.1
additional information
Homo sapiens
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Bos taurus
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Leishmania infantum
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
?
-
-
-
7.6.2.1
additional information
Saccharomyces cerevisiae
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
?
-
-
-
7.6.2.1
phospholipid/in
Homo sapiens
unspecific bidirectional transmembrane transport of phospholipids
phospholipid/out
-
-
r
7.6.2.1
phospholipid/in
Rattus norvegicus
unspecific bidirectional transmembrane transport of phospholipids
phospholipid/out
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.1
ATP8A1 from chromaffin granules
Bos taurus
7.6.2.1
partially from erythrocytes
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.1
blood platelet
-
Homo sapiens
-
7.6.2.1
endothelial cell
-
Homo sapiens
-
7.6.2.1
erythrocyte
-
Homo sapiens
-
7.6.2.1
fibroblast
-
Homo sapiens
-
7.6.2.1
liver
-
Rattus norvegicus
-
7.6.2.1
osteoblast
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Homo sapiens
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Saccharomyces cerevisiae
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Bos taurus
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Leishmania infantum
ADP + phosphate + phosphatidylethanolamine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Homo sapiens
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Saccharomyces cerevisiae
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Bos taurus
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane
668247
Leishmania infantum
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/out
transport from the exoplasmic to the cytosolic leaflet of the plasma membrane, and from the lumenal side of granules to the external side
668247
Bos taurus
ADP + phosphate + phosphatidylserine/in
-
-
-
?
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Bos taurus
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane
668247
Leishmania infantum
?
-
-
-
-
7.6.2.1
additional information
the enzyme is a P-type ATPase involved in regulation of the lipid asymmetry of the cell membrane, especially Neo1p is important, Drs2p is involved in vesicle formation in the Golgi and thus influences the plasma membrane distribution of aminophospholipids despite its localization the Golgi membrane, mechanism
668247
Saccharomyces cerevisiae
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Saccharomyces cerevisiae
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Bos taurus
?
-
-
-
-
7.6.2.1
additional information
the enzyme depends on ATP
668247
Leishmania infantum
?
-
-
-
-
7.6.2.1
additional information
the enzyme is independent of ATP or energy supply
668247
Homo sapiens
?
-
-
-
-
7.6.2.1
additional information
the enzyme is independent of ATP or energy supply
668247
Rattus norvegicus
?
-
-
-
-
7.6.2.1
phospholipid/in
unspecific bidirectional transmembrane transport of phospholipids
668247
Homo sapiens
phospholipid/out
-
-
-
r
7.6.2.1
phospholipid/in
unspecific bidirectional transmembrane transport of phospholipids
668247
Rattus norvegicus
phospholipid/out
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.6.2.1
?
x * 37000, scramblase
Homo sapiens