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Literature summary extracted from

  • Bellemare, V.; Faucher, F.; Breton, R.; Luu-The, V.
    Characterization of 17alpha-hydroxysteroid dehydrogenase activity (17alpha-HSD) and its involvement in the biosynthesis of epitestosterone (2005), BMC Biochem., 6, 12.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.148 cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.148 additional information Mus musculus the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.148 Mus musculus Q91WR5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.148 recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.148 kidney high expression level Mus musculus
-
1.1.1.148 additional information enzyme tissue distribution in male and female mice, overview Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.148 5alpha-dihydrotestosterone + NADPH
-
 Mus musculus 5alpha-androstane-3alpha,17alpha-diol + NADP+
-
 ?
1.1.1.148 5alpha-pregnane-3,20-dione + NADPH + H+
-
 Mus musculus 3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
-
 ?
1.1.1.148 androstanedione + NADPH
-
 Mus musculus 5alpha-androstane-3-one-17alpha-ol + NADP+
-
 ?
1.1.1.148 androstenedione + NADPH + H+
-
 Mus musculus epitestosterone + NADP+
-
 ?
1.1.1.148 androsterone + NADPH
-
 Mus musculus 5alpha-androstane-3alpha,17alpha-diol + NADP+
-
 ?
1.1.1.148 dehydroepiandrosterone + NADPH
-
 Mus musculus 5-androstene-3alpha,17alpha-diol + NADP+
-
 ?
1.1.1.148 additional information the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone Mus musculus ?
-
 ?
1.1.1.148 additional information substrate specificity of the recombinant enzyme, overview Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.148 17alpha-HSD
-
 Mus musculus
1.1.1.148 17alpha-hydroxysteroid dehydrogenase
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.148 7.3
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.148 NADPH
-
 Mus musculus