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Literature summary extracted from

  • Ichinose, H.; Kamiya, N.; Goto, M.
    Enzymatic redox cofactor regeneration in organic media: functionalization and application of glycerol dehydrogenase and soluble transhydrogenase in reverse micelles (2005), Biotechnol. Prog., 21, 1192-1197.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 ammonium sulfate about 100% activation at 10 mM Escherichia coli
1.1.1.6 K+ activates NADH production Escherichia coli
1.1.1.6 NH4+ activates NADH production Escherichia coli

Application

EC Number Application Comment Organism
1.1.1.6 molecular biology enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview Escherichia coli
1.6.1.1 biotechnology enzymatic NADH production system in reverse micelles using a bacterial glycerol dehydrogenase. The present system is further extended to NADPH production in reverse micelles by coupling with a bacterial soluble transhydrogenase that catalyses the conversion of NADP+ to NADPH using NADH. Glycerol dehydrogenase and soluble transhydrogenase have potential for use in redox cofactor recycling in reverse micelles, which allows the use of catalytic quantities of NAD(P)H in organic media Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.6 expression as His-tag fusion protein in Escherichia coli BL21 (DE3) pLysS Escherichia coli
1.1.1.6 gene gld, expression of His-tagged enzyme in strain BL21(DE3) Escherichia coli
1.1.99.22 expression in Escherichia coli BL21 as His-tag fusion protein Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 NH4+ 80% activation at 10 mM Escherichia coli
1.1.99.22 NH4+ 85% activation at 10 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Escherichia coli
-
 D-glyceraldehyde + NADH + H+
-
 r
1.1.1.6 glycerol + NAD+ Escherichia coli JM109
-
 D-glyceraldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Escherichia coli
-
-
-
1.1.1.6 Escherichia coli
-
 gene gld
-
1.1.1.6 Escherichia coli JM109
-
 gene gld
-
1.1.99.22 Escherichia coli
-
-
-
1.1.99.22 Escherichia coli JM109
-
-
-
1.6.1.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6 purified by Ni-affinity chromatography Escherichia coli
1.1.1.6 recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli
1.1.99.22 to homogeneity by Ni-affinity chromatography Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.6 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 additional information
-
 assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli JM109 dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Escherichia coli D-glyceraldehyde + NADH + H+
-
 r
1.1.1.6 glycerol + NAD+
-
 Escherichia coli JM109 D-glyceraldehyde + NADH + H+
-
 r
1.1.99.22 glycerol + NAD+
-
 Escherichia coli dihydroxyacetone + NADH + H+
-
 ?
1.1.99.22 glycerol + NAD+
-
 Escherichia coli JM109 dihydroxyacetone + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.6 GLD
-
 Escherichia coli
1.1.1.6 glycerol dehydrogenase
-
 Escherichia coli
1.1.99.22 GLD
-
 Escherichia coli
1.1.99.22 glycerol dehydrogenase
-
 Escherichia coli
1.6.1.1 soluble transhydrogenase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 37
-
 assay at, in reverse micelles Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 9.5
-
 in an aqueous buffer system Escherichia coli
1.1.1.6 10.5
-
 in reverse micelles Escherichia coli
1.1.1.6 10.5 11 NADH production Escherichia coli
1.1.99.22 9.5
-
 in an aqueous buffer system Escherichia coli
1.1.99.22 10.5
-
 in reverse micelles Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.6 6 11
-
 Escherichia coli
1.1.99.22 6 11
-
 Escherichia coli
1.6.1.1 7 10.5 pH 7.0: about 55% of maximal activity, pH 10.5: about 45% of maximal activity Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 additional information no activity with NADP+ and NADPH Escherichia coli
1.1.1.6 NAD+
-
 Escherichia coli
1.1.1.6 NADH
-
 Escherichia coli