EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.6 | ammonium sulfate | about 100% activation at 10 mM | Escherichia coli | |
1.1.1.6 | K+ | activates NADH production | Escherichia coli | |
1.1.1.6 | NH4+ | activates NADH production | Escherichia coli |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.6 | molecular biology | enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview | Escherichia coli |
1.6.1.1 | biotechnology | enzymatic NADH production system in reverse micelles using a bacterial glycerol dehydrogenase. The present system is further extended to NADPH production in reverse micelles by coupling with a bacterial soluble transhydrogenase that catalyses the conversion of NADP+ to NADPH using NADH. Glycerol dehydrogenase and soluble transhydrogenase have potential for use in redox cofactor recycling in reverse micelles, which allows the use of catalytic quantities of NAD(P)H in organic media | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.6 | expression as His-tag fusion protein in Escherichia coli BL21 (DE3) pLysS | Escherichia coli |
1.1.1.6 | gene gld, expression of His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
1.1.99.22 | expression in Escherichia coli BL21 as His-tag fusion protein | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.6 | NH4+ | 80% activation at 10 mM | Escherichia coli | |
1.1.99.22 | NH4+ | 85% activation at 10 mM | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.6 | glycerol + NAD+ | Escherichia coli | - |
D-glyceraldehyde + NADH + H+ | - |
r | |
1.1.1.6 | glycerol + NAD+ | Escherichia coli JM109 | - |
D-glyceraldehyde + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.6 | Escherichia coli | - |
- |
- |
1.1.1.6 | Escherichia coli | - |
gene gld | - |
1.1.1.6 | Escherichia coli JM109 | - |
gene gld | - |
1.1.99.22 | Escherichia coli | - |
- |
- |
1.1.99.22 | Escherichia coli JM109 | - |
- |
- |
1.6.1.1 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.6 | purified by Ni-affinity chromatography | Escherichia coli |
1.1.1.6 | recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
1.1.99.22 | to homogeneity by Ni-affinity chromatography | Escherichia coli |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.6 | cell culture | - |
Escherichia coli | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | additional information | - |
assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.6 | glycerol + NAD+ | the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate | Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? | |
1.1.1.6 | glycerol + NAD+ | the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate | Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? | |
1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli | D-glyceraldehyde + NADH + H+ | - |
r | |
1.1.1.6 | glycerol + NAD+ | - |
Escherichia coli JM109 | D-glyceraldehyde + NADH + H+ | - |
r | |
1.1.99.22 | glycerol + NAD+ | - |
Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? | |
1.1.99.22 | glycerol + NAD+ | - |
Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.6 | GLD | - |
Escherichia coli |
1.1.1.6 | glycerol dehydrogenase | - |
Escherichia coli |
1.1.99.22 | GLD | - |
Escherichia coli |
1.1.99.22 | glycerol dehydrogenase | - |
Escherichia coli |
1.6.1.1 | soluble transhydrogenase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 37 | - |
assay at, in reverse micelles | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 9.5 | - |
in an aqueous buffer system | Escherichia coli |
1.1.1.6 | 10.5 | - |
in reverse micelles | Escherichia coli |
1.1.1.6 | 10.5 | 11 | NADH production | Escherichia coli |
1.1.99.22 | 9.5 | - |
in an aqueous buffer system | Escherichia coli |
1.1.99.22 | 10.5 | - |
in reverse micelles | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.6 | 6 | 11 | - |
Escherichia coli |
1.1.99.22 | 6 | 11 | - |
Escherichia coli |
1.6.1.1 | 7 | 10.5 | pH 7.0: about 55% of maximal activity, pH 10.5: about 45% of maximal activity | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.6 | additional information | no activity with NADP+ and NADPH | Escherichia coli | |
1.1.1.6 | NAD+ | - |
Escherichia coli | |
1.1.1.6 | NADH | - |
Escherichia coli |