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Literature summary extracted from

  • Zhang, J.F.; Shi, L.Y.; Wei, D.Z.
    Chemical modification of L-asparaginase from Escherichia coli with a modified polyethyleneglycol under substrate protection conditions (2004), Biotechnol. Lett., 26, 753-756.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine the enzyme is a potential therapeutic agent in acute lymphocytic leukemia, acute lymphoblastic leukemia, and chromic myelogenyous leukemia, but causes high allergic reactions Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 additional information reduction of the allergenic potential of the enzyme as therapeutic agent by chemical modification of the enzyme with 2,4-bis(O-methoxypolyethyleneglycol)-6-chloro-S-triazine, mPEG2, in presence of L-asparagine, optimally with a mPEG2/-NH2 molar ratio of 10, the modified enzyme retains 33% of initial enzymatic activity with complete abolishment of immunogenicity, in vitro half-life increments from 4.6 h to 33 h is obtained, method overview Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Escherichia coli
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Escherichia coli L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7
-
 assay at Escherichia coli