EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.2 | analysis | the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Levilactobacillus brevis |
1.1.1.2 | analysis | the enzyme is utilized in a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Thermoanaerobacter brockii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.2 | additional information | evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Levilactobacillus brevis |
1.1.1.2 | additional information | evaluation of a dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid, overview | Thermoanaerobacter brockii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | 12-ketochenodeoxycholic acid | inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially | Levilactobacillus brevis | |
1.1.1.2 | acetone | at high concentrations of e.g. 3.4 M | Levilactobacillus brevis | |
1.1.1.2 | acetone | at high concentrations of e.g. 3.4 M | Thermoanaerobacter brockii | |
1.1.1.2 | cholic acid | inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially | Levilactobacillus brevis | |
1.1.1.176 | acetone | at 25% v/v and above | Clostridium sp. | |
1.1.1.176 | additional information | no substrate inhibition at high concentration of cholic acid | Clostridium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 2-propanol + NADP+ | Levilactobacillus brevis | - |
acetone + NADPH | - |
r | |
1.1.1.2 | 2-propanol + NADP+ | Thermoanaerobacter brockii | - |
acetone + NADPH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Levilactobacillus brevis | - |
- |
- |
1.1.1.2 | Thermoanaerobacter brockii | - |
- |
- |
1.1.1.176 | Clostridium sp. | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.2 | commercial preparation | - |
Levilactobacillus brevis | - |
1.1.1.2 | commercial preparation | - |
Thermoanaerobacter brockii | - |
1.1.1.176 | commercial preparation | enzyme in 50% glycerol-containing solution | Clostridium sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.176 | additional information | - |
assay method development using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, optimization, overview | Clostridium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.2 | 1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, 51% and 46% residual activity, respectively | Levilactobacillus brevis |
1.1.1.2 | 1 unit of ADH dissolved in 0.5 ml of 0.1 M potassium phosphate buffer, pH 8.0, containing 4% w/v cholic acid 100 mM, 100 mM NADP+, 1 mM dithiothreitol, and 10% or 25% v/v acetone, 23 days, quite stable | Thermoanaerobacter brockii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 2-propanol + NADP+ | - |
Levilactobacillus brevis | acetone + NADPH | - |
r | |
1.1.1.2 | 2-propanol + NADP+ | - |
Thermoanaerobacter brockii | acetone + NADPH | - |
r | |
1.1.1.176 | cholic acid + NADP+ | the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview | Clostridium sp. | 12-ketochenodeoxycholic acid + NADPH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | ADH | - |
Levilactobacillus brevis |
1.1.1.2 | ADH | - |
Thermoanaerobacter brockii |
1.1.1.176 | 12alpha-HSDH | - |
Clostridium sp. |
1.1.1.176 | NADP-dependent 12alpha-hydroxysteroid dehydrogenase | - |
Clostridium sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 22 | - |
assay at room temperature | Levilactobacillus brevis |
1.1.1.2 | 22 | - |
assay at room temperature | Thermoanaerobacter brockii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 8 | - |
assay at | Levilactobacillus brevis |
1.1.1.2 | 8 | - |
assay at | Thermoanaerobacter brockii |
1.1.1.176 | 8 | - |
- |
Clostridium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | NADP+ | dependent on | Levilactobacillus brevis | |
1.1.1.2 | NADP+ | dependent on | Thermoanaerobacter brockii | |
1.1.1.2 | NADPH | dependent on | Levilactobacillus brevis | |
1.1.1.2 | NADPH | dependent on | Thermoanaerobacter brockii | |
1.1.1.176 | NADP+ | - |
Clostridium sp. |