Literature summary extracted from

Kim, S.H.; Zhang, J.; Jiang, Y.; Zhou, H.M.; Yan, Y.B.
Assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclodextrins (2006), Biophys. J., 91, 686-693.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.14	Sus scrofa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.14	native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration	Sus scrofa

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
3.5.1.14	assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclodextrins in a concentration-dependent manner, most efficiently by hydroxypropyl beta-cyclodextrin, low concentrations of hydroxypropyl alpha-cyclodextrin and high concentrations of hydroxypropyl gamma-cyclodextrin promoted off-pathway aggregation, recovery of secondary and tertiary structures, overview	Sus scrofa

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.5.1.14	kidney	-	Sus scrofa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.14	chloroacetyl-L-leucine + H2O	-	Sus scrofa	chloroacetate + L-leucine	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.5.1.14	25	-	assay at	Sus scrofa

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Release 2023.1 (January 2023)