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Literature summary extracted from

  • Kishi, T.; Cloutier, S.M.; Kuendig, C.; Deperthes, D.; Diamandis, E.P.
    Activation and enzymatic characterization of recombinant human kallikrein 8 (2006), Biol. Chem., 387, 723-731.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.118 antipain 50% inhibition at 0.00046 mM Homo sapiens
3.4.21.118 chymostatin 50% inhibition at 0.008 mM Homo sapiens
3.4.21.118 leupeptin 50% inhibition at 0.066 mM Homo sapiens
3.4.21.118 Ni2+ 50% inhibition at 0.011 mM Homo sapiens
3.4.21.118 Zn2+ 50% inhibition at 0.0033 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.118 Ca2+ 4fold stimulation Homo sapiens
3.4.21.118 K+ 1.7fold stimulation Homo sapiens
3.4.21.118 Mg2+ 3foldstimulation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.118 Homo sapiens O60259 recombinant enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.118 proteolytic modification pro-enzyme may be activiated by lysyl-endopeptidase by cleavage of a 9 amino acid propeptide Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.118 benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 1000 M/s Homo sapiens benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 1000 M/s Homo sapiens benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 additional information enzyme shows trypsin-like specificity. Synthetic substrates containing either Arg or Lys at P1 positions are cleaved Homo sapiens ?
-
 ?
3.4.21.118 t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 21000 M/s Homo sapiens t-butyloxycarbonyl-L-Val-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.118 tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 670 M/s Homo sapiens tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.118 8.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.118 5.5
-
 no detectable activity below Homo sapiens
3.4.21.118 7.4 10 more than 50% of maximum activity within this range Homo sapiens