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Literature summary extracted from

  • Asensio, A.C.; Rodriguez-Ferrer, C.R.; Oaknin, S.; Rotllan, P.
    Biochemical and immunochemical characterisation of human diadenosine triphosphatase provides evidence for its identification with the tumour suppressor Fhit protein (2006), Biochimie, 88, 461-471.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.29 diethyl dicarbonate inactivation Homo sapiens
3.6.1.29 suramin strong, competitive inhibition, IC50: 0.002 mM Homo sapiens
3.6.1.29 Zn2+ IC50: 0.02 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.29 0.0014
-
 P1,P3-bis(5'-adenosyl)triphosphate pH 7.0, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.29 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.29 Ca2+ maximal activation at 0.5-1.0 mM Homo sapiens
3.6.1.29 Mg2+ dependent on, maximal activation at 0.5-1.0 mM Homo sapiens
3.6.1.29 Mn2+ maximal activation at 0.5-1.0 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.29 17000
-
 2 * 17000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O Homo sapiens
-
 ADP + AMP
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O Rattus norvegicus
-
 ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.29 Homo sapiens
-
-
-
3.6.1.29 Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.29 native enzyme from blood platelets and leukocytes, 19000fold, by anion exchange chromatography and N6-5'-AMP affinity chromatography to homogeneity Homo sapiens
3.6.1.29 native enzyme from brain cytosolic fraction by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and N6-5'-AMP affinity chromatography to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.29 blood platelet
-
 Homo sapiens
-
3.6.1.29 brain
-
 Rattus norvegicus
-
3.6.1.29 leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.29 1.34
-
 purified native enzyme from leukocytes Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O
-
 Homo sapiens ADP + AMP
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl)triphosphate + H2O
-
 Rattus norvegicus ADP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.29 dimer 2 * 17000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.29 diadenosine triphosphatase
-
 Homo sapiens
3.6.1.29 diadenosine triphosphatase
-
 Rattus norvegicus
3.6.1.29 tumour suppressor Fhit protein
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.29 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.29 7 7.2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.29 0.000022
-
 suramin pH 7.0, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.29 0.002
-
 strong, competitive inhibition, IC50: 0.002 mM Homo sapiens suramin
3.6.1.29 0.02
-
 IC50: 0.02 mM Homo sapiens Zn2+