EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.102 | gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) | Candida albicans |
1.1.1.102 | gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3) | Aspergillus fumigatus |
1.1.1.102 | gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.102 | additional information | construction of an enzyme-deficient mutant by gene replacement, the mutant produces lower levels of inositolphosphorylceramides | Candida albicans |
1.1.1.102 | additional information | construction of the deletion mutant TscDELTA38p | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.102 | additional information | - |
additional information | binding kinetics of substrate analogues with shorter chain lengths | Candida albicans | |
1.1.1.102 | 0.009 | - |
3-dehydrosphinganine | pH 7.5, recombinant enzyme | Candida albicans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.102 | 3-dehydrosphinganine + NADPH | Aspergillus fumigatus | - |
sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | Saccharomyces cerevisiae | the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview | sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | Candida albicans | the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides | sphinganine + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.102 | Aspergillus fumigatus | - |
gene ksrA | - |
1.1.1.102 | Candida albicans | - |
gene ksr1 | - |
1.1.1.102 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.102 | 3-dehydrosphinganine + NADPH | - |
Saccharomyces cerevisiae | sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | - |
Aspergillus fumigatus | sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview | Saccharomyces cerevisiae | sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides | Candida albicans | sphinganine + NADP+ | - |
? | |
1.1.1.102 | 3-dehydrosphinganine + NADPH | C18 substrate, molecular modeling of substrate binding, overview | Candida albicans | sphinganine + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.102 | More | molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview | Candida albicans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.102 | 3-ketosphinganine reductase | - |
Saccharomyces cerevisiae |
1.1.1.102 | 3-ketosphinganine reductase | - |
Candida albicans |
1.1.1.102 | 3-ketosphinganine reductase | - |
Aspergillus fumigatus |
1.1.1.102 | Ksr1p | - |
Candida albicans |
1.1.1.102 | KsrA | - |
Aspergillus fumigatus |
1.1.1.102 | More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Saccharomyces cerevisiae |
1.1.1.102 | More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Candida albicans |
1.1.1.102 | More | the enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes | Aspergillus fumigatus |
1.1.1.102 | TSC10 | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.102 | 1.07 | - |
3-dehydrosphinganine | pH 7.5, recombinant enzyme | Candida albicans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.102 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
1.1.1.102 | 7.5 | - |
assay at | Candida albicans |
1.1.1.102 | 7.5 | - |
assay at | Aspergillus fumigatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.102 | NADPH | - |
Saccharomyces cerevisiae | |
1.1.1.102 | NADPH | - |
Candida albicans | |
1.1.1.102 | NADPH | - |
Aspergillus fumigatus |