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Literature summary extracted from

  • Kappler, U.; Bailey, S.; Feng, C.; Honeychurch, M.J.; Hanson, G.R.; Bernhardt, P.V.; Tollin, G.; Enemark, J.H.
    Kinetic and structural evidence for the importance of Tyr236 for the integrity of the Mo active site in a bacterial sulfite dehydrogenase (2006), Biochemistry, 45, 9696-9705.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.1 expressed in Escherichia coli strain DH5alpha Starkeya novella

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.2.1
-
 Starkeya novella

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.1 Y236F reduced turnover rates and substrate affinity as well as an altered reactivity toward molecular oxygen as an electron acceptor, unlike the wild type enzyme the mutant enzyme is reoxidized quickly in the presence of molecular oxygen Starkeya novella

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Molybdenum
-
 Starkeya novella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 42000
-
 wild type enzyme, analytical ultracentrifugation Starkeya novella
1.8.2.1 43000
-
 mutant enzyme Y236F, analytical ultracentrifugation Starkeya novella

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1 DEAE anion exchange column chromatography Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.1 heterodimer
-
 Starkeya novella

Synonyms

EC Number Synonyms Comment Organism
1.8.2.1 SDH
-
 Starkeya novella

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.2.1 0.4
-
 sulfite mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella
1.8.2.1 0.8
-
 sulfite mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
1.8.2.1 33.51
-
 sulfite wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
1.8.2.1 36.19
-
 sulfite mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 Starkeya novella
1.8.2.1 52.51
-
 sulfite mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella
1.8.2.1 333.7
-
 sulfite wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0 Starkeya novella

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.1 5.5 9.4 wild type enzyme Starkeya novella
1.8.2.1 7.2 8.64 mutant enzyme Y236F Starkeya novella

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.1 heme c
-
 Starkeya novella