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Literature summary extracted from

  • Stone, E.M.; Costello, A.L.; Tierney, D.L.; Fast, W.
    Substrate-assisted cysteine deprotonation in the mechanism of dimethylargininase (DDAH) from Pseudomonas aeruginosa (2006), Biochemistry, 45, 5618-5630.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.18 C249S catalytically inactive Pseudomonas aeruginosa
3.5.3.18 C249S active-site Cys249 residue Pseudomonas aeruginosa
3.5.3.18 H162G catalytically inactive Pseudomonas aeruginosa
3.5.3.18 H162G mutation of the active-site histidine residue Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.18 iodoacetamide pH dependent DDAH inactivation, addition of 2.5 mM NG-methyl-L-arginine at pH 8.5 can prevent inactivation by idoacetamide, inactivation may be due to modification at the active site of DDAH, inactivation increases at higher pH values Pseudomonas aeruginosa
3.5.3.18 L-lysine competitive inhibitor; potential competitive inhibitor Pseudomonas aeruginosa
3.5.3.18 Zinc acetate
-
 Pseudomonas aeruginosa
3.5.3.18 Zn2+
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.18 0.026
-
 S-methyl-L-thiocitrulline at pH 8.0 Pseudomonas aeruginosa
3.5.3.18 0.039
-
 NG,NG-dimethyl-L-arginine at pH 8.0 Pseudomonas aeruginosa
3.5.3.18 0.044
-
 NG-methyl-L-arginine at pH 8.0 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.18 30420
-
 mass spectrometry, mutant H162G Pseudomonas aeruginosa
3.5.3.18 30500
-
 electrospray ionization mass spectrometry Pseudomonas aeruginosa
3.5.3.18 30500
-
 mass spectrometry, wilde type DDAH Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.18 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.18
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O
-
 Pseudomonas aeruginosa dimethylamine + L-citrulline
-
 ?
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O
-
 Pseudomonas aeruginosa L-citrulline + dimethylamine
-
 ?
3.5.3.18 NG-methyl-L-arginine + H2O
-
 Pseudomonas aeruginosa L-citrulline + methylamine
-
 ?
3.5.3.18 S-methyl-L-thiocitrulline + H2O
-
 Pseudomonas aeruginosa methanethiol + L-citrulline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.18 DDAH
-
 Pseudomonas aeruginosa
3.5.3.18 dimethylarginine dimethylaminohydrolase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.18 0.59
-
 NG-methyl-L-arginine at pH 8.0 Pseudomonas aeruginosa
3.5.3.18 1.27
-
 NG,NG-dimethyl-L-arginine at pH 8.0 Pseudomonas aeruginosa
3.5.3.18 1.3
-
 S-methyl-L-thiocitrulline at pH 8.0 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.18 4.5 9.5
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.18 0.4
-
 L-lysine at pH 7.3 Pseudomonas aeruginosa
3.5.3.18 4
-
 L-lysine at pH 7.3 Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.18 0.0088
-
-
 Pseudomonas aeruginosa Zinc acetate