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Literature summary extracted from

  • Kim, H.Y.; Gladyshev, V.N.
    Role of structural and functional elements of mouse methionine-S-sulfoxide reductase in its subcellular distribution (2005), Biochemistry, 44, 8059-8067.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 msrA, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged or GFP-tagged wild-type or truncated MsrA in Escherichia coli strain BL21(DE3), expression of MsrA isozymes in Saccharomyces cerevisiae, subcellular localization of the recombinant enzymes in cytosol and mitochondria, overview Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 additional information construction of the truncated MsrA DELTA(1-46) mutant Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 0.34
-
 dabsyl-L-methionine-(S)-S-oxide recombinant wild-type MsrA, with DTT, pH 7.5, 37°C Mus musculus
1.8.4.11 4.3
-
 dabsyl-L-methionine-(S)-S-oxide recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.11 cytosol
-
 Mus musculus 5829
-
1.8.4.11 mitochondrion
-
 Mus musculus 5739
-
1.8.4.11 additional information post-translationally occuring subcellular targeting and distribution of isozymes, overview, several forms are generated from a single translation form Mus musculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin Mus musculus MsrA is involved in repair of oxidized proteins peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 0.099
-
 purified recombinant truncated MsrA DELTA(1-46), substrates DTT and dabsyl-L-methionine-(S)-S-oxide Mus musculus
1.8.4.11 0.238
-
 purified recombinant wild-type MsrA, substrates DTT and dabsyl-L-methionine-(S)-S-oxide Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 dabsyl-L-methionine-(S)-S-oxide + DTT stereospecific reduction Mus musculus dabsyl-L-methionine + DTT disulfide + H2O
-
 ?
1.8.4.11 additional information role of subcellular localization in structure-function relationship of the isozymes, overview Mus musculus ?
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction Mus musculus peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin MsrA is involved in repair of oxidized proteins Mus musculus peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine-S-sulfoxide reductase
-
 Mus musculus
1.8.4.11 MsrA
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 37
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 0.28
-
 dabsyl-L-methionine-(S)-S-oxide recombinant wild-type MsrA, with DTT, pH 7.5, 37°C Mus musculus
1.8.4.11 0.78
-
 dabsyl-L-methionine-(S)-S-oxide recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.5
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 dithiothreitol
-
 Mus musculus
1.8.4.11 thioredoxin
-
 Mus musculus