EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.7 | expressed in Escherichia coli | Pyrococcus horikoshii |
3.6.1.7 | expression in Escherichia coli | Pyrococcus horikoshii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.7 | sitting-drop-vapor-diffusion method, 1.5 A crystal structure. The enzyme forms a dimer in the crystal structure via antiparallel association of strand 4 | Pyrococcus horikoshii |
3.6.1.7 | sitting-drop-vapor-diffusion with sodium formate as precipitant at pH 6.0 | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.7 | 0.12 | - |
benzoyl phosphate | at 25°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 0.12 | - |
benzoyl phosphate | 25-45°C, pH 5.3 | Pyrococcus horikoshii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.7 | 10128 | - |
1 * 10128, enzyme in solution | Pyrococcus horikoshii |
3.6.1.7 | 10128 | - |
2 * 10128, in the crystal structure via antiparallel association of strand 4 with formation of nine interchain hydrogen bonds | Pyrococcus horikoshii |
3.6.1.7 | 10129 | - |
1 * 10129, calculated from sequence, although the enzyme exists as a monomer in solution, it can dimerize via antiparallel association of strand 4, the protein forms a dimer in the crystal structure via antiparallel association of strand 4 | Pyrococcus horikoshii |
3.6.1.7 | 10130 | - |
mass spectrometry | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.7 | Pyrococcus horikoshii | P84142 | - |
- |
3.6.1.7 | Pyrococcus horikoshii DSM 12428 | P84142 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.7 | - |
Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.7 | 1,3-diphosphoglycerate + H2O | - |
Pyrococcus horikoshii | 3-phosphoglycerate + phosphate | - |
? | |
3.6.1.7 | 1,3-diphosphoglycerate + H2O | - |
Pyrococcus horikoshii DSM 12428 | 3-phosphoglycerate + phosphate | - |
? | |
3.6.1.7 | acetyl phosphate + H2O | - |
Pyrococcus horikoshii | acetate + phosphate | - |
? | |
3.6.1.7 | acetyl phosphate + H2O | - |
Pyrococcus horikoshii DSM 12428 | acetate + phosphate | - |
? | |
3.6.1.7 | benzoyl phosphate + H2O | - |
Pyrococcus horikoshii | benzoate + phosphate | - |
? | |
3.6.1.7 | benzoyl phosphate + H2O | - |
Pyrococcus horikoshii DSM 12428 | benzoate + phosphate | - |
? | |
3.6.1.7 | carbamoyl phosphate + H2O | - |
Pyrococcus horikoshii | carbamate + phosphate | - |
? | |
3.6.1.7 | carbamoyl phosphate + H2O | - |
Pyrococcus horikoshii DSM 12428 | carbamate + phosphate | - |
? | |
3.6.1.7 | nucleoside diphosphate + H2O | - |
Pyrococcus horikoshii | nucleoside phosphate + phosphate | - |
? | |
3.6.1.7 | nucleoside diphosphate + H2O | - |
Pyrococcus horikoshii DSM 12428 | nucleoside phosphate + phosphate | - |
? | |
3.6.1.7 | nucleoside triphosphate + H2O | - |
Pyrococcus horikoshii | nucleoside diphosphate + phosphate | - |
? | |
3.6.1.7 | succinyl phosphate + H2O | - |
Pyrococcus horikoshii | succinate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.7 | dimer | 2 * 10128, in the crystal structure via antiparallel association of strand 4 with formation of nine interchain hydrogen bonds | Pyrococcus horikoshii |
3.6.1.7 | monomer | 1 * 10128, enzyme in solution | Pyrococcus horikoshii |
3.6.1.7 | monomer | 1 * 10129, calculated from sequence, although the enzyme exists as a monomer in solution, it can dimerize via antiparallel association of strand 4, the protein forms a dimer in the crystal structure via antiparallel association of strand 4 | Pyrococcus horikoshii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.7 | PhAcP | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.7 | 98 | - |
- |
Pyrococcus horikoshii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.7 | 25 | 45 | turnover number increases 4fold by an increase of temperature from 25 to 45°C | Pyrococcus horikoshii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.7 | 25 | 100 | At 25°C, the free energy of unfolding, midpoint of transition, and m value were 54.4 kJ/mol, 4.99 M, and 10.9 kJ/mol/M, respectively. The melting temperature is about 111.5°C. These data indicate that PhAcP is an extremely thermostable protein. | Pyrococcus horikoshii |
3.6.1.7 | 100 | - |
stable up to | Pyrococcus horikoshii |
3.6.1.7 | 112 | - |
melting temperature: 111.5°C | Pyrococcus horikoshii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.7 | additional information | - |
additional information | experimentally, it is impossible to assay the enzyme activity of the enzyme directly at 98°C because the uncatalyzed hydrolysis of the acyl-phosphate substrate is too fast at high temperatures to allow an accurate measurement of kinetic parameters. Instead, the kinetic parameters of the enzyme are measured at 25-45 °C and the kcat value of the enzyme at 98 °C is estimated by linear extrapolation of the Arrhenius plot. The estimated kcat value is 10000/s | Pyrococcus horikoshii | |
3.6.1.7 | 93.5 | - |
benzoyl phosphate | at 25°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 93.5 | - |
benzoyl phosphate | 25°C, pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 191 | - |
benzoyl phosphate | at 30°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 191 | - |
benzoyl phosphate | 30°C, pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 275 | - |
benzoyl phosphate | at 35°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 275 | - |
benzoyl phosphate | 35°C, pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 290 | - |
benzoyl phosphate | at 40°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 290 | - |
benzoyl phosphate | 40°C, pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 467 | - |
benzoyl phosphate | at 45°C and pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 467 | - |
benzoyl phosphate | 45°C, pH 5.3 | Pyrococcus horikoshii | |
3.6.1.7 | 10000 | - |
benzoyl phosphate | at 98°C and pH 5.3, estimation by linear extrapolation | Pyrococcus horikoshii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.7 | 5.3 | - |
- |
Pyrococcus horikoshii |
3.6.1.7 | 5.3 | - |
at 25°C | Pyrococcus horikoshii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.7 | 779 | - |
benzoyl phosphate | 25 °C, pH 5.3 | Pyrococcus horikoshii |