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Literature summary extracted from

  • Polticelli, F.; Zaini, G.; Bolli, A.; Antonini, G.; Gradoni, L.; Ascenzi, P.
    Probing the cruzain S2 recognition subsite: a kinetic and binding energy calculation study (2005), Biochemistry, 44, 2781-2789.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.51 0.0021
-
 N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi
3.4.22.51 0.0021
-
 N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi
3.4.22.51 0.0025
-
 N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.51 Trypanosoma cruzi
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.51 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.51 N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
-
 Trypanosoma cruzi N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.22.51 N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
-
 Trypanosoma cruzi N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.22.51 N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine-(7-amido-4-methylcoumarin) + H2O
-
 Trypanosoma cruzi N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.51 0.13
-
 N-alpha-benzyloxycarbonyl-L-tyrosyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi
3.4.22.51 0.14
-
 N-alpha-benzyloxy-carbonyl-L-phenylalanyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi
3.4.22.51 0.15
-
 N-alpha-benzyloxycarbonyl-L-arginyl-L-alanine-(7-amido-4-methylcoumarin) 37°C, pH 6.5 Trypanosoma cruzi