BRENDA - Enzyme Database

Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER

Chang, Q.L.; Gummadi, S.N.; Menon, A.K.; Biochemistry 43, 10710-10718 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
diethyldicarbonate
rat liver contains two populations, DEPC-sensitive and DEPC-insensitive, of flippases, which can be in a different of DEPC sensitivity or be two different proteins
Rattus norvegicus
7.6.2.1
NEM
rat liver contains two populations of, NEM-sensitive and NEM-insensitive, flippases, which can be in a different of NEM sensitivity or be two different proteins
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
endoplasmic reticulum membrane
-
Rattus norvegicus
5789
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
additional information
Rattus norvegicus
the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process
?
-
-
-
7.6.2.1
phospholipid/in
Rattus norvegicus
-
phospholipid/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.1
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.6.2.1
native enzyme from liver endoplasmic reticulum
Rattus norvegicus
Renatured (Commentary)
EC Number
Commentary
Organism
7.6.2.1
reconstitution of the purified native enzyme in proteoliposomes using phospholipids and Triton X-100 at pH 7.5 and 22°C
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.1
additional information
-
aminophospholipid translocase assay using dithionite reduction by 4-nitrobenzo-2-oxa-1,3-diazole-labeled phospholipids, dithionate does not permeate membranes
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
additional information
the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process
667578
Rattus norvegicus
?
-
-
-
-
7.6.2.1
phosphatidylcholine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylcholine/out
-
-
-
?
7.6.2.1
phosphatidylethanolamine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylethanolemine/out
-
-
-
?
7.6.2.1
phosphatidylserine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylserine/out
-
-
-
?
7.6.2.1
phospholipid/in
-
667578
Rattus norvegicus
phospholipid/out
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.1
22
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.1
7.5
-
assay at
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.6.2.1
diethyldicarbonate
rat liver contains two populations, DEPC-sensitive and DEPC-insensitive, of flippases, which can be in a different of DEPC sensitivity or be two different proteins
Rattus norvegicus
7.6.2.1
NEM
rat liver contains two populations of, NEM-sensitive and NEM-insensitive, flippases, which can be in a different of NEM sensitivity or be two different proteins
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.6.2.1
endoplasmic reticulum membrane
-
Rattus norvegicus
5789
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.6.2.1
additional information
Rattus norvegicus
the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process
?
-
-
-
7.6.2.1
phospholipid/in
Rattus norvegicus
-
phospholipid/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.1
native enzyme from liver endoplasmic reticulum
Rattus norvegicus
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
7.6.2.1
reconstitution of the purified native enzyme in proteoliposomes using phospholipids and Triton X-100 at pH 7.5 and 22°C
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.6.2.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.6.2.1
additional information
-
aminophospholipid translocase assay using dithionite reduction by 4-nitrobenzo-2-oxa-1,3-diazole-labeled phospholipids, dithionate does not permeate membranes
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
additional information
the enzyme is a key enzyme of membrane biogenesis performing transbilayer flipping of glycerophospholipids in the endoplasmic reticulum, this flipping is an ATP-independent, bidirectional process
667578
Rattus norvegicus
?
-
-
-
-
7.6.2.1
phosphatidylcholine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylcholine/out
-
-
-
?
7.6.2.1
phosphatidylethanolamine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylethanolemine/out
-
-
-
?
7.6.2.1
phosphatidylserine/in
proteoliposomes generated from aminophospholipid translocase-enriched Triton X-100 extract of endoplasmic reticulum
667578
Rattus norvegicus
phosphatidylserine/out
-
-
-
?
7.6.2.1
phospholipid/in
-
667578
Rattus norvegicus
phospholipid/out
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
7.6.2.1
22
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6.2.1
7.5
-
assay at
Rattus norvegicus