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Literature summary extracted from

  • Gallego, O.; Belyaeva, O.V.; Porte, S.; Ruiz, F.X.; Stetsenko, A.V.; Shabrova, E.V.; Kostereva, N.V.; Farres, J.; Pares, X.; Kedishvili, N.Y.
    Comparative functional analysis of human medium-chain dehydrogenases, short-chain dehydrogenases/reductases and aldo-keto reductases with retinoids (2006), Biochem. J., 399, 101-109.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.105
-
Homo sapiens
1.1.1.300
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.105 0.0011
-
all-trans-retinol 37°C Homo sapiens
1.1.1.300 0.18
-
all-trans-retinol 37°C Homo sapiens
1.1.1.300 0.3
-
all-trans-retinal 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.300 membrane microsomal membrane Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.105 all-trans-retinol + NAD+ Homo sapiens RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context all-trans-retinal + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Homo sapiens O75452
-
-
1.1.1.300 Homo sapiens Q8TC12
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.105 preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel Homo sapiens
1.1.1.300 purified to homogeneity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 all-trans-retinol + NAD+ RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context Homo sapiens all-trans-retinal + NADH
-
?
1.1.1.105 all-trans-retinol + NAD+
-
Homo sapiens all-trans-retinal + NADH + H+
-
?
1.1.1.105 additional information the enzymes utilizes retinol bound to cellular retinol binding protein type I at a much lower rate than free retinol Homo sapiens ?
-
?
1.1.1.300 all-trans-retinol + NADP+ more efficient in the reductive direction Homo sapiens all-trans-retinal + NADPH + H+
-
r
1.1.1.300 additional information the enzymes utilizes retinol bound to cellular retinol binding protein type I at a much lower rate than free retinol Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 RoDH-4
-
Homo sapiens
1.1.1.300 RDH11
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.105 0.02
-
all-trans-retinol 37°C Homo sapiens
1.1.1.300 0.00012
-
all-trans-retinal 37°C Homo sapiens
1.1.1.300 0.0006
-
all-trans-retinol 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 NAD+ preferred cofactor Homo sapiens
1.1.1.300 NADP+ preferred cofactor Homo sapiens
1.1.1.300 NADPH preferred cofactor Homo sapiens