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Literature summary extracted from

  • Gul, S.; Mellor, G.W.; Thomas, E.W.; Brocklehurst, K.
    Temperature-dependences of the kinetics of reactions of papain and actinidin with a series of reactivity probes differing in key molecular recognition features (2006), Biochem. J., 396, 17-21.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 additional information
-
 additional information kinetics, stopped-flow method using interaction of the enzyme with seven different 2-pyridyl disulfide reactivity probes, overview Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-
3.4.22.14 Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 CH3-CH2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-(D-Phe)-NH-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-(D-Phe)-O-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-(L-Phe)-NH-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-(L-Phe)-O-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-NH-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 CH3CO-O-[CH2]2-2-pyridyl disulfide + H2O
-
 Carica papaya ?
-
 ?
3.4.22.2 additional information activation reaction of the enzyme with seven different substrate-derived 2-pyridyl disulfide reactivity probes, specificity, overview Carica papaya ?
-
 ?
3.4.22.14 2-(acetamido)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 2-(acetoxy)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 2-(N'-acetyl-D-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 2-(N'-acetyl-D-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 2-(N'-acetyl-L-phenylalanyl)hydroxyethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 2-(N'-acetyl-L-phenylalanylamino)ethylene 2'-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?
3.4.22.14 ethyl 2-pyridyl disulfide + H2O reactivity probe, analysis of temperature-dependence of reaction kinetics Actinidia chinensis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 additional information
-
 temperature-dependences of the second-order rate constants of the reaction involving the catalytic site thiol, overview, stopped-flow method Carica papaya

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.2 4 30
-
 Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6
-
 about Carica papaya