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Literature summary extracted from

  • Reid, J.D.; Hussain, S.; Bailey, T.S.; Sonkaria, S.; Sreedharan, S.K.; Thomas, E.W.; Resmini, M.; Brocklehurst, K.
    Isomerization of the uncomplexed actinidin molecule: kinetic accessibility of additional steps in enzyme catalysis provided by solvent perturbation (2004), Biochem. J., 378, 699-703.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.14 0.25
-
 N-acetyl-L-Phe-Gly methyl ester above 0.25 mM, at 4.3% acetonitrile, 25°C, pH 5.3, parameter for pre-steady-state acylation phase Actinidia chinensis
3.4.22.14 0.543
-
 N-acetyl-L-Phe-Gly methyl ester 25°C, pH 5.3, 8.3% acetonitrile parameter for pre-steady-state acylation phase Actinidia chinensis
3.4.22.14 0.893
-
 N-acetyl-L-Phe-Gly methyl ester 25°C, pH 5.3, 16.7% acetonitrile, parameter for pre-steady-state acylation phase Actinidia chinensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.14 acetonitrile stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics. With low acetonitrile content, plots of reaction are linear, increasing the acetonitrile content results in marked deviations of the plots from linearity with a rate minimum around [S]0 of 0.15 mM Actinidia chinensis
3.4.22.14 dimethyl sulfoxide comparison of effect on reaction kinetics with acetonitrile Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 N-acetyl-L-Phe-Gly methyl ester + H2O stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics Actinidia chinensis ?
-
 ?