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Literature summary extracted from
- Role of osmolytes as chemical chaperones during the refolding of aminoacylase (2006), Biochem. Cell Biol., 84, 30-38.
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.14 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | native enzyme from kidney by acetone precipitation, ion exchange chromatography, and gel filtration | Sus scrofa |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.5.1.14 | 60fold dilution of guanidinum hydrochloride-denatured enzyme, role of osmolytes, e.g. glycerol, DMSO, sucrose, or proline, as chemical chaperones during the refolding of aminoacylase, off-pathway aggregation occurs, osmolytes inhibits aggregation and recover enzyme activity during refolding, overview | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.14 | kidney | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.14 | chloroacetyl-L-leucine + H2O | - |
Sus scrofa | chloroacetate + L-leucine | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.14 | 25 | - |
assay at | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.14 | 7.5 | - |
assay at | Sus scrofa |
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Release 2023.1 (January 2023)
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