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Literature summary extracted from

  • Kinouchi, T.; Ishiura, S.; Mabuchi, Y.; Urakami-Manaka, Y.; Nishio, H.; Nishiuchi, Y.; Tsunemi, M.; Takada, K.; Watanabe, M.; Ikeda, M.; Matsui, H.; Tomioka, S.; Kawahara, H.; Hamamoto, T.; Suzuki, K.; Kagawa, Y.
    Mammalian D-aspartyl endopeptidase: a scavenger for noxious racemized proteins in aging (2004), Biochem. Biophys. Res. Commun., 314, 730-736.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.99.B2 1,10-phenanthroline 0.1 mM, 8.3% inhibition Oryctolagus cuniculus
3.4.99.B2 Aprotinin 0.1 mM, 14% inhibition Oryctolagus cuniculus
3.4.99.B2 benzoyl-L-Arg-L-His-D-Asp-CH2Cl 0.003 mM, 50% inhibition Oryctolagus cuniculus
3.4.99.B2 diisopropyl fluorophosphate at 0.5 mM, 15.5% inhibition Oryctolagus cuniculus
3.4.99.B2 lactacystin
-
Mus musculus
3.4.99.B2 lactacystin
-
Oryctolagus cuniculus
3.4.99.B2 leupeptin 0.03 mM, 8.5% inhibition Oryctolagus cuniculus
3.4.99.B2 additional information no inhibition: N-p-tosyl-L-phenylalanine chloromethyl ketone, N-p-tosyl-L-lysine chloromethyl ketone, phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane, L-alanyl-L-alanyl-L-phenylalanyl-chloromethane, chymostatin, EDTA, epoxomicin, N-acetyl-L-leucyl-L-leucyl-norleucinal Oryctolagus cuniculus
3.4.99.B2 pepstatin A 0.01 mM,5.3% inhibition Oryctolagus cuniculus
3.4.99.B2 phosphoramidon 0.01 mM, 9.9% inhibition Oryctolagus cuniculus
3.4.99.B2 SDS 0.01% Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.99.B2 0.12
-
succinyl-D-Asp-alpha-4-methylcoumaryl-7-amide pH 8,5, 37°C Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.99.B2 mitochondrial inner membrane
-
Mus musculus 5743
-
3.4.99.B2 mitochondrial inner membrane
-
Oryctolagus cuniculus 5743
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.99.B2 Ba2+ 3 mM, activity is increased by a factor of two Oryctolagus cuniculus
3.4.99.B2 Ca2+ 3 mM, activity is increased by a factor of two Oryctolagus cuniculus
3.4.99.B2 Mg2+ 3 mM, activity is increased by a factor of two Oryctolagus cuniculus
3.4.99.B2 Mn2+ 3 mM, activity is increased by a factor of two Oryctolagus cuniculus
3.4.99.B2 Sr2+ 3 mM, activity is increased by a factor of two Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.99.B2 600000
-
multi-complex structure Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.99.B2 additional information Oryctolagus cuniculus the accumulation of D-isomers of aspartic acid in proteins during aging is involved in the pathogenesis of Alzheimer’s disease, cataracts, and arteriosclerosis. The enzyme may serve as a scavenger against accumulation of racemized proteins in aging ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.99.B2 Mus musculus
-
-
-
3.4.99.B2 no activity in Caenorhabditis elegans
-
-
-
3.4.99.B2 no activity in Escherichia coli
-
-
-
3.4.99.B2 no activity in Saccharomyces cerevisiae
-
-
-
3.4.99.B2 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.99.B2
-
Mus musculus
3.4.99.B2
-
Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.99.B2 brain weak activity Oryctolagus cuniculus
-
3.4.99.B2 kidney
-
Oryctolagus cuniculus
-
3.4.99.B2 liver
-
Mus musculus
-
3.4.99.B2 liver
-
Oryctolagus cuniculus
-
3.4.99.B2 lung moderate activity Oryctolagus cuniculus
-
3.4.99.B2 ovary weak activity Oryctolagus cuniculus
-
3.4.99.B2 small intestine moderate activity Oryctolagus cuniculus
-
3.4.99.B2 spleen
-
Oryctolagus cuniculus
-
3.4.99.B2 stomach weak activity Oryctolagus cuniculus
-
3.4.99.B2 testis weak activity Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.99.B2 additional information the accumulation of D-isomers of aspartic acid in proteins during aging is involved in the pathogenesis of Alzheimer’s disease, cataracts, and arteriosclerosis. The enzyme may serve as a scavenger against accumulation of racemized proteins in aging Oryctolagus cuniculus ?
-
?
3.4.99.B2 additional information no activity with acetyl-Tyr-Val-Ala-Asp-4-methylcoumaryl-7-amide, acetyl-Asp-Glu-Val-Asp-4-methylcoumaryl-7-amide, succinyl-Gly-Pro-Leu-Gly-Pro-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide, Leu-4-methylcoumaryl-7-amide, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Leu-Leu-Glu-4-methylcoumaryl-7-amide, Ala-Ala-Phe-4-methylcoumaryl-7-amide, succinyl-Ala-[D-Ser]-4-methylcoumaryl-7-amide, succinyl-[D-Ser]-4-methylcoumaryl-7-amide Oryctolagus cuniculus ?
-
?
3.4.99.B2 succinyl-D-Asp-alpha-4-methylcoumaryl-7-amide + H2O
-
Oryctolagus cuniculus succinyl-D-Asp + 7-amino-4-methylcoumarin
-
?
3.4.99.B2 [D-Asp]AEFRH[D-Asp]SGY + H2O the D-Abeta1-10 peptide is degraded at D-Asp7 and two peptides are produced [D-Asp]AEFRHX and SGY. X is not identified in this study. L-Abeta1–10 peptide could not be degraded by this enzyme Oryctolagus cuniculus [D-Asp]AEFRHX + SGY
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.99.B2 D-aspartyl endopeptidase
-
Mus musculus
3.4.99.B2 D-aspartyl endopeptidase
-
Oryctolagus cuniculus
3.4.99.B2 DAEP
-
Mus musculus
3.4.99.B2 DAEP
-
Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.99.B2 8.5
-
-
Oryctolagus cuniculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.99.B2 0.003
-
pH 8.5, 37°C Oryctolagus cuniculus benzoyl-L-Arg-L-His-D-Asp-CH2Cl
3.4.99.B2 0.15
-
-
Oryctolagus cuniculus lactacystin