EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.99.B2 | 1,10-phenanthroline | 0.1 mM, 8.3% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | Aprotinin | 0.1 mM, 14% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | benzoyl-L-Arg-L-His-D-Asp-CH2Cl | 0.003 mM, 50% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | diisopropyl fluorophosphate | at 0.5 mM, 15.5% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | lactacystin | - |
Mus musculus | |
3.4.99.B2 | lactacystin | - |
Oryctolagus cuniculus | |
3.4.99.B2 | leupeptin | 0.03 mM, 8.5% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | additional information | no inhibition: N-p-tosyl-L-phenylalanine chloromethyl ketone, N-p-tosyl-L-lysine chloromethyl ketone, phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane, L-alanyl-L-alanyl-L-phenylalanyl-chloromethane, chymostatin, EDTA, epoxomicin, N-acetyl-L-leucyl-L-leucyl-norleucinal | Oryctolagus cuniculus | |
3.4.99.B2 | pepstatin A | 0.01 mM,5.3% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | phosphoramidon | 0.01 mM, 9.9% inhibition | Oryctolagus cuniculus | |
3.4.99.B2 | SDS | 0.01% | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.99.B2 | 0.12 | - |
succinyl-D-Asp-alpha-4-methylcoumaryl-7-amide | pH 8,5, 37°C | Oryctolagus cuniculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.99.B2 | mitochondrial inner membrane | - |
Mus musculus | 5743 | - |
3.4.99.B2 | mitochondrial inner membrane | - |
Oryctolagus cuniculus | 5743 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.99.B2 | Ba2+ | 3 mM, activity is increased by a factor of two | Oryctolagus cuniculus | |
3.4.99.B2 | Ca2+ | 3 mM, activity is increased by a factor of two | Oryctolagus cuniculus | |
3.4.99.B2 | Mg2+ | 3 mM, activity is increased by a factor of two | Oryctolagus cuniculus | |
3.4.99.B2 | Mn2+ | 3 mM, activity is increased by a factor of two | Oryctolagus cuniculus | |
3.4.99.B2 | Sr2+ | 3 mM, activity is increased by a factor of two | Oryctolagus cuniculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.99.B2 | 600000 | - |
multi-complex structure | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.99.B2 | additional information | Oryctolagus cuniculus | the accumulation of D-isomers of aspartic acid in proteins during aging is involved in the pathogenesis of Alzheimers disease, cataracts, and arteriosclerosis. The enzyme may serve as a scavenger against accumulation of racemized proteins in aging | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.99.B2 | Mus musculus | - |
- |
- |
3.4.99.B2 | no activity in Caenorhabditis elegans | - |
- |
- |
3.4.99.B2 | no activity in Escherichia coli | - |
- |
- |
3.4.99.B2 | no activity in Saccharomyces cerevisiae | - |
- |
- |
3.4.99.B2 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.99.B2 | - |
Mus musculus |
3.4.99.B2 | - |
Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.99.B2 | brain | weak activity | Oryctolagus cuniculus | - |
3.4.99.B2 | kidney | - |
Oryctolagus cuniculus | - |
3.4.99.B2 | liver | - |
Mus musculus | - |
3.4.99.B2 | liver | - |
Oryctolagus cuniculus | - |
3.4.99.B2 | lung | moderate activity | Oryctolagus cuniculus | - |
3.4.99.B2 | ovary | weak activity | Oryctolagus cuniculus | - |
3.4.99.B2 | small intestine | moderate activity | Oryctolagus cuniculus | - |
3.4.99.B2 | spleen | - |
Oryctolagus cuniculus | - |
3.4.99.B2 | stomach | weak activity | Oryctolagus cuniculus | - |
3.4.99.B2 | testis | weak activity | Oryctolagus cuniculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.99.B2 | additional information | the accumulation of D-isomers of aspartic acid in proteins during aging is involved in the pathogenesis of Alzheimers disease, cataracts, and arteriosclerosis. The enzyme may serve as a scavenger against accumulation of racemized proteins in aging | Oryctolagus cuniculus | ? | - |
? | |
3.4.99.B2 | additional information | no activity with acetyl-Tyr-Val-Ala-Asp-4-methylcoumaryl-7-amide, acetyl-Asp-Glu-Val-Asp-4-methylcoumaryl-7-amide, succinyl-Gly-Pro-Leu-Gly-Pro-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide, Leu-4-methylcoumaryl-7-amide, succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide, benzyloxycarbonyl-Leu-Leu-Glu-4-methylcoumaryl-7-amide, Ala-Ala-Phe-4-methylcoumaryl-7-amide, succinyl-Ala-[D-Ser]-4-methylcoumaryl-7-amide, succinyl-[D-Ser]-4-methylcoumaryl-7-amide | Oryctolagus cuniculus | ? | - |
? | |
3.4.99.B2 | succinyl-D-Asp-alpha-4-methylcoumaryl-7-amide + H2O | - |
Oryctolagus cuniculus | succinyl-D-Asp + 7-amino-4-methylcoumarin | - |
? | |
3.4.99.B2 | [D-Asp]AEFRH[D-Asp]SGY + H2O | the D-Abeta1-10 peptide is degraded at D-Asp7 and two peptides are produced [D-Asp]AEFRHX and SGY. X is not identified in this study. L-Abeta110 peptide could not be degraded by this enzyme | Oryctolagus cuniculus | [D-Asp]AEFRHX + SGY | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.99.B2 | D-aspartyl endopeptidase | - |
Mus musculus |
3.4.99.B2 | D-aspartyl endopeptidase | - |
Oryctolagus cuniculus |
3.4.99.B2 | DAEP | - |
Mus musculus |
3.4.99.B2 | DAEP | - |
Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.99.B2 | 8.5 | - |
- |
Oryctolagus cuniculus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.4.99.B2 | 0.003 | - |
pH 8.5, 37°C | Oryctolagus cuniculus | benzoyl-L-Arg-L-His-D-Asp-CH2Cl | |
3.4.99.B2 | 0.15 | - |
- |
Oryctolagus cuniculus | lactacystin |