Literature summary extracted from

Kong, X.; Liu, Y.; Gou, X.; Zhu, S.; Zhang, H.; Wang, X.; Zhang, J.
Directed evolution of alpha-aspartyl dipeptidase from Salmonella typhimurium (2001), Biochem. Biophys. Res. Commun., 289, 137-142.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.13.21	N89H/Q153E/L205R	47fold increase in enzyme activity, increased stability	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.13.21	2.26	-	L-Asp-4-nitroanilide	mutant N89H/Q153E/L205R	Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21	3.98	-	L-Asp-4-nitroanilide	wild-type	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.13.21	Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.13.21	L-Asp-4-nitroanilide + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	L-Asp + 4-nitroaniline	-	?

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.4.13.21	60	-	30 min, 25% residual activity for wild-type, 80% residual activity for mutant N89H/Q153E/L205R	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.13.21	0.22	-	L-Asp-4-nitroanilide	wild-type	Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21	5.83	-	L-Asp-4-nitroanilide	mutant N89H/Q153E/L205R	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.13.21	7	-	mutant N89H/Q153E/L205R	Salmonella enterica subsp. enterica serovar Typhimurium
3.4.13.21	7.2	-	wild-type	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)