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Literature summary extracted from

  • Niu, Q.; Huang, X.; Zhang, L.; Li, Y.; Li, J.; Yang, J.; Zhang, K.
    A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes (2006), Arch. Microbiol., 185, 439-448.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.28 Al3+
-
 Bacillus nematocida
3.4.24.28 dithiothreitol 5 mM, 83% residual activity Bacillus nematocida
3.4.24.28 Hg2+
-
 Bacillus nematocida
3.4.24.28 phenylmethylsulfonyl fluoride 0.1 mM, 77% residual activity Bacillus nematocida
3.4.24.28 Zn2+
-
 Bacillus nematocida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.28 extracellular
-
 Bacillus nematocida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.28 Ca2+ 100 mM, 60% activation Bacillus nematocida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.28 40000
-
 x * 40000, SDS-PAGE Bacillus nematocida

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.28 Bacillus nematocida
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.28 55.6
-
 pH 6.5, 55°C Bacillus nematocida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.28 Bovine serum albumin + H2O 8.7% of the activity with denatured casein Bacillus nematocida ?
-
 ?
3.4.24.28 Collagen + H2O 1.3% of the activity with denatured casein Bacillus nematocida ?
-
 ?
3.4.24.28 Denatured casein + H2O
-
 Bacillus nematocida ?
-
 ?
3.4.24.28 denatured collagen + H2O 11.5% of the activity with denatured casein Bacillus nematocida ?
-
 ?
3.4.24.28 Gelatin + H2O 9% of the activity with denatured casein Bacillus nematocida ?
-
 ?
3.4.24.28 nematode cuticle + H2O 2.8% of the activity with denatured casein Bacillus nematocida ?
-
 ?
3.4.24.28 skimmed milk + H2O 60% of the activity with denatured casein Bacillus nematocida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.28 ? x * 40000, SDS-PAGE Bacillus nematocida

Synonyms

EC Number Synonyms Comment Organism
3.4.24.28 Bae16
-
 Bacillus nematocida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.28 55
-
-
 Bacillus nematocida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.28 30 60 stable for 30 min Bacillus nematocida
3.4.24.28 70
-
 1 h, complete inactivation Bacillus nematocida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.28 6.5
-
-
 Bacillus nematocida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.28 6.5 11 decrease of activity from pH 6.5 to 11 Bacillus nematocida
3.4.24.28 11
-
 no residual activity Bacillus nematocida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.28 4
-
 1h, less than 40% remaining activity Bacillus nematocida
3.4.24.28 5 8 stable within this range Bacillus nematocida