Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yokochi, N.; Nishimura, S.; Yoshikane, Y.; Ohnishi, K.; Yagi, T.
    Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti (2006), Arch. Biochem. Biophys., 452, 1-8.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.107 expression in Escherichia coli Mesorhizobium loti
1.1.1.107 recombinant enzyme expressed in Escherichia coli Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.107 0.091
-
pyridoxal pH 9.2, 30°C Mesorhizobium loti
1.1.1.107 0.091
-
pyridoxal 30°C, pH 9.2 Mesorhizobium loti
1.1.1.107 0.28
-
NAD+ pH 9.2, 30°C Mesorhizobium loti
1.1.1.107 0.28
-
NAD+ 30°C, pH 9.2 Mesorhizobium loti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.107 25531
-
4 * 25531, calculated from sequence Mesorhizobium loti
1.1.1.107 26000
-
4 * 26000, SDS-PAGE Mesorhizobium loti
1.1.1.107 26000
-
4 * 26000 Mesorhizobium loti
1.1.1.107 97000
-
gel filtration Mesorhizobium loti
1.1.1.107 98000
-
non-denaturing PAGE Mesorhizobium loti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.107 pyridoxal + NAD+ Mesorhizobium loti PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine 4-pyridoxolactone + NADH + H+
-
?
1.1.1.107 pyridoxal + NAD+ Mesorhizobium loti PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway 4-pyridoxolactone + NADH + H+
-
ir
1.1.1.107 pyridoxal + NAD+ Mesorhizobium loti MAFF303099 PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine 4-pyridoxolactone + NADH + H+
-
?
1.1.1.107 pyridoxal + NAD+ Mesorhizobium loti MAFF303099 PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway 4-pyridoxolactone + NADH + H+
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.107 Mesorhizobium loti
-
-
-
1.1.1.107 Mesorhizobium loti
-
MAFF303099
-
1.1.1.107 Mesorhizobium loti MAFF303099
-
MAFF303099
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.107 recombinant Mesorhizobium loti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.107 88
-
-
Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.107 additional information the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) Mesorhizobium loti ?
-
?
1.1.1.107 additional information the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) Mesorhizobium loti MAFF303099 ?
-
?
1.1.1.107 pyridoxal + NAD+ PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
?
1.1.1.107 pyridoxal + NAD+ PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
ir
1.1.1.107 pyridoxal + NAD+ very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
ir
1.1.1.107 pyridoxal + NAD+ PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
?
1.1.1.107 pyridoxal + NAD+ PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
ir
1.1.1.107 pyridoxal + NAD+ very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
ir

Subunits

EC Number Subunits Comment Organism
1.1.1.107 tetramer 4 * 26000, SDS-PAGE Mesorhizobium loti
1.1.1.107 tetramer 4 * 26000 Mesorhizobium loti
1.1.1.107 tetramer 4 * 25531, calculated from sequence Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
1.1.1.107 PLDH
-
Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.107 50
-
-
Mesorhizobium loti

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.107 30 55 30°C: 37% of maximal activity, 55°C: 33% of maximal activity Mesorhizobium loti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.107 45
-
10 min, stable below Mesorhizobium loti
1.1.1.107 50
-
10 min, 76% of original activity Mesorhizobium loti
1.1.1.107 60
-
10 min, 1.3% of original activity Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.107 136
-
NAD+ pH 9.2, 30°C Mesorhizobium loti
1.1.1.107 136
-
NAD+ 30°C, pH 9.2 Mesorhizobium loti
1.1.1.107 149
-
pyridoxal pH 9.2, 30°C Mesorhizobium loti
1.1.1.107 149
-
pyridoxal 30°C, pH 9.2 Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.107 9.2
-
-
Mesorhizobium loti
1.1.1.107 9.2
-
50 mM CHES-NaOH buffer Mesorhizobium loti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.107 8.6 10 pH 8.6: 66% of maximal activity, pH 10.0: 57% of maximal activity, 50 mM CHES-NaOH buffer Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.107 NAD+
-
Mesorhizobium loti