EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.107 | expression in Escherichia coli | Mesorhizobium loti |
1.1.1.107 | recombinant enzyme expressed in Escherichia coli | Mesorhizobium loti |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.107 | 0.091 | - |
pyridoxal | pH 9.2, 30°C | Mesorhizobium loti | |
1.1.1.107 | 0.091 | - |
pyridoxal | 30°C, pH 9.2 | Mesorhizobium loti | |
1.1.1.107 | 0.28 | - |
NAD+ | pH 9.2, 30°C | Mesorhizobium loti | |
1.1.1.107 | 0.28 | - |
NAD+ | 30°C, pH 9.2 | Mesorhizobium loti |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 25531 | - |
4 * 25531, calculated from sequence | Mesorhizobium loti |
1.1.1.107 | 26000 | - |
4 * 26000, SDS-PAGE | Mesorhizobium loti |
1.1.1.107 | 26000 | - |
4 * 26000 | Mesorhizobium loti |
1.1.1.107 | 97000 | - |
gel filtration | Mesorhizobium loti |
1.1.1.107 | 98000 | - |
non-denaturing PAGE | Mesorhizobium loti |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.107 | pyridoxal + NAD+ | Mesorhizobium loti | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | 4-pyridoxolactone + NADH + H+ | - |
? | |
1.1.1.107 | pyridoxal + NAD+ | Mesorhizobium loti | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | 4-pyridoxolactone + NADH + H+ | - |
ir | |
1.1.1.107 | pyridoxal + NAD+ | Mesorhizobium loti MAFF303099 | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | 4-pyridoxolactone + NADH + H+ | - |
? | |
1.1.1.107 | pyridoxal + NAD+ | Mesorhizobium loti MAFF303099 | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | 4-pyridoxolactone + NADH + H+ | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.107 | Mesorhizobium loti | - |
- |
- |
1.1.1.107 | Mesorhizobium loti | - |
MAFF303099 | - |
1.1.1.107 | Mesorhizobium loti MAFF303099 | - |
MAFF303099 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.107 | recombinant | Mesorhizobium loti |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 88 | - |
- |
Mesorhizobium loti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.107 | additional information | the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) | Mesorhizobium loti | ? | - |
? | |
1.1.1.107 | additional information | the enzyme shows less than 0.01% of the activity of pyridoxal toward pyridoxal 5'-phosphate (0.5 mM), pyridoxine (0.8 mM), and pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, pyridine, formaldehyde, and 2-carboxybenzaldehyde (1.0 mM each). The enzyme shows less than 0.01% of the activity of pyridoxal toward DL-glyceraldehyde, D-xylose, L-xylose, D-ribose, D-glucose, D-arabinose, L-arabinose, L-fucose, and D-galactose (250 mM each) | Mesorhizobium loti MAFF303099 | ? | - |
? | |
1.1.1.107 | pyridoxal + NAD+ | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
? | |
1.1.1.107 | pyridoxal + NAD+ | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
ir | |
1.1.1.107 | pyridoxal + NAD+ | very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction | Mesorhizobium loti | 4-pyridoxolactone + NADH + H+ | - |
ir | |
1.1.1.107 | pyridoxal + NAD+ | PLDH is essential for assimilation of vitamin B6 compounds and is the second step enzyme in the degradation pathway for pyridoxine | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
? | |
1.1.1.107 | pyridoxal + NAD+ | PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
ir | |
1.1.1.107 | pyridoxal + NAD+ | very low activity toward NADP+. 0.16% of the maximal activity obtained with NAD+ is observed when 5.0 mM NADP+ is used. The enzyme does not catalyze the reduction of 4-pyridoxolactone with NADH + H+ to pyridoxal even when a 100fold higher concentration of the enzyme is used for the reverse reaction | Mesorhizobium loti MAFF303099 | 4-pyridoxolactone + NADH + H+ | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.107 | tetramer | 4 * 26000, SDS-PAGE | Mesorhizobium loti |
1.1.1.107 | tetramer | 4 * 26000 | Mesorhizobium loti |
1.1.1.107 | tetramer | 4 * 25531, calculated from sequence | Mesorhizobium loti |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.107 | PLDH | - |
Mesorhizobium loti |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 50 | - |
- |
Mesorhizobium loti |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 30 | 55 | 30°C: 37% of maximal activity, 55°C: 33% of maximal activity | Mesorhizobium loti |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 45 | - |
10 min, stable below | Mesorhizobium loti |
1.1.1.107 | 50 | - |
10 min, 76% of original activity | Mesorhizobium loti |
1.1.1.107 | 60 | - |
10 min, 1.3% of original activity | Mesorhizobium loti |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.107 | 136 | - |
NAD+ | pH 9.2, 30°C | Mesorhizobium loti | |
1.1.1.107 | 136 | - |
NAD+ | 30°C, pH 9.2 | Mesorhizobium loti | |
1.1.1.107 | 149 | - |
pyridoxal | pH 9.2, 30°C | Mesorhizobium loti | |
1.1.1.107 | 149 | - |
pyridoxal | 30°C, pH 9.2 | Mesorhizobium loti |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 9.2 | - |
- |
Mesorhizobium loti |
1.1.1.107 | 9.2 | - |
50 mM CHES-NaOH buffer | Mesorhizobium loti |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.107 | 8.6 | 10 | pH 8.6: 66% of maximal activity, pH 10.0: 57% of maximal activity, 50 mM CHES-NaOH buffer | Mesorhizobium loti |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.107 | NAD+ | - |
Mesorhizobium loti |